Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution
Open Access
- 8 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), srep01804-6
- https://doi.org/10.1038/srep01804
Abstract
Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.This publication has 34 references indexed in Scilit:
- Virtual nanoscopy: Generation of ultra-large high resolution electron microscopy mapsThe Journal of cell biology, 2012
- TrakEM2 Software for Neural Circuit ReconstructionPLOS ONE, 2012
- Intravital imaging of CTLs killing islet cells in diabetic miceJCI Insight, 2012
- Current views on ljungan virus and its relationship to human diabetesJournal of Medical Virology, 2011
- National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2·7 million participantsThe Lancet, 2011
- Restoration of impaired intestinal barrier function by the hydrolysed casein diet contributes to the prevention of type 1 diabetes in the diabetes-prone BioBreeding ratDiabetologia, 2010
- Quantitative analysis of cell composition and purity of human pancreatic islet preparationsLaboratory Investigation, 2010
- Antiviral treatments reduce severity of diabetes in Ljungan virus-infected CD-1 mice and delay onset in diabetes-prone BB ratsMicrobiology and Immunology, 2009
- Analysis of islet inflammation in human type 1 diabetesClinical and Experimental Immunology, 2008
- Coxsackie B4 virus infection of β cells and natural killer cell insulitis in recent-onset type 1 diabetic patientsProceedings of the National Academy of Sciences of the United States of America, 2007