Influence of Anthocyanins, Derivative Pigments and Other Catechol and Pyrogallol-Type Phenolics on Breast Cancer Cell Proliferation
- 19 February 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (6), 3785-3792
- https://doi.org/10.1021/jf903714z
Abstract
Anthocyanins (cyanidin-3-glucoside (Cy-3-gluc) and delphinidin-3-glucoside (Dp-3-gluc)) and their respective vinylpyranoanthocyanin-catechins (portisins) were studied in order to evaluate the cytotoxicity effect on the estrogen responsive human breast cancer cell line (ER+) MCF-7 and their effect on estrogen receptor (ER-α and ER-β) expression. Other flavonoid classes and phenolic molecules were also tested, aiming to study possible structural features related with these effects. Also, the antiproliferative effect of Cy-3-gluc and Dp-3-gluc was studied by an immunofluorescence assay. Generally, all the anthocyanin pigments studied inhibited, in a dose-dependent manner, the growth of the (ER+) MCF-7. The cytotoxicity effect was higher when cells were treated with Dp-3-gluc and its respective portisin. Altogether, the results point to the ortho trihydroxylated moiety in the phenolic ring as an important structural feature for more potent cytotoxicity effect on MCF-7 cells comparatively to the effect observed with the similar dihydroxylated compounds. In order to elucidate the molecular mechanism involved, expression of estrogen receptor was assayed by RT-PCR and real time RT-PCR. The higher antiproliferative effect observed after cell treatment with Dp-3-gluc was not followed by modification on ER expression. However, the anthocyanin Cy-3-gluc was able to induce a downregulation of ER levels although with no significant effect on MCF-7 proliferation.This publication has 44 references indexed in Scilit:
- Delphinidin inhibits cell proliferation and invasion via modulation of Met receptor phosphorylationToxicology and Applied Pharmacology, 2008
- Color Properties of Four Cyanidin−Pyruvic Acid AdductsJournal of Agricultural and Food Chemistry, 2006
- Com-1/P8 in oestrogen regulated growth of breast cancer cells, the ER-β connectionBiochemical and Biophysical Research Communications, 2005
- Human tumor cell growth inhibition by nontoxic anthocyanidins, the pigments in fruits and vegetablesLife Sciences, 2005
- Anthocyanidins inhibit activator protein 1 activity and cell transformation: structure-activity relationship and molecular mechanismsCarcinogenesis: Integrative Cancer Research, 2003
- Anthocyanin-derived pigments and colour of red winesAnalytica Chimica Acta, 2002
- Anthocyanins and other flavonoids (January 1998 to December 2000)Natural Product Reports, 2001
- Antioxidant and Prooxidant Behavior of Flavonoids: Structure-Activity RelationshipsFree Radical Biology & Medicine, 1997
- Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cellsNutrition and Cancer, 1997
- New Colorimetric Cytotoxicity Assay for Anticancer-Drug ScreeningJNCI Journal of the National Cancer Institute, 1990