Smc5/6 maintains stalled replication forks in a recombination-competent conformation
- 21 January 2009
- journal article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 28 (2), 144-155
- https://doi.org/10.1038/emboj.2008.273
Abstract
The Smc5/6 structural maintenance of chromosomes complex is required for efficient homologous recombination (HR). Defects in Smc5/6 result in chromosome mis‐segregation and fragmentation. By characterising two Schizosaccharomyces pombe smc6 mutants, we define two separate functions for Smc5/6 in HR. The first represents the previously described defect in processing recombination‐dependent DNA intermediates when replication forks collapse, which leads to increased rDNA recombination. The second novel function defines Smc5/6 as a positive regulator of recombination in the rDNA and correlates mechanistically with a requirement to load RPA and Rad52 onto chromatin genome‐wide when replication forks are stably stalled by nucleotide depletion. Rad52 is required for all HR repair, but Rad52 loading in response to replication fork stalling is unexpected and does not correlate with damage‐induced foci. We propose that Smc5/6 is required to maintain stalled forks in a stable recombination‐competent conformation primed for replication restart.Keywords
This publication has 56 references indexed in Scilit:
- Activation of Ubiquitin-Dependent DNA Damage Bypass Is Mediated by Replication Protein AMolecular Cell, 2008
- Regulation of Rtt107 Recruitment to Stalled DNA Replication Forks by the Cullin Rtt101 and the Rtt109 AcetyltransferaseMolecular Biology of the Cell, 2008
- RecQ helicases queuing with Srs2 to disrupt Rad51 filaments and suppress recombinationJournal of Bone and Joint Surgery, 2007
- Replication in Hydroxyurea: It's a Matter of TimeMolecular and Cellular Biology, 2007
- Brc1-Mediated Rescue of Smc5/6 Deficiency: Requirement for Multiple Nucleases and a Novel Rad18 FunctionGenetics, 2007
- Genome-wide localization of pre-RC sites and identification of replication origins in fission yeastThe EMBO Journal, 2007
- Smc5/6 Is Required for Repair at Collapsed Replication ForksMolecular and Cellular Biology, 2006
- Postreplication Repair and PCNA Modification inSchizosaccharomyces pombeMolecular Biology of the Cell, 2006
- DNA Replication Origins Fire Stochastically in Fission YeastMolecular Biology of the Cell, 2006
- [56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombeMethods in Enzymology, 1991