Flavonoid isolation and identification of mother-in-law’s tongue leaves (sansevieria trifasciata) and the inhibitory activities to xanthine oxidase enzyme
Open Access
- 26 November 2018
- journal article
- research article
- Published by EDP Sciences in E3S Web of Conferences
- Vol. 67, 03011
- https://doi.org/10.1051/e3sconf/20186703011
Abstract
Hyperuricemia is a disease that is characterized by high uric acid levels, in which the number of victim increase year by year in the worldwide. Flavonoid is an active compound with inhibitory activity towards Xanthine Oxidase Enzyme which is a compound that plays a role in the formation of uric acid in the body. Sansevieria trifasciata is an ornamental plant which is also useful as a source of antibacterial and antioxidant agent. Studies of S. trifasciata as Xanthine Oxidase Enzyme inhibitor have not been reported. This research isolate flavonoid compounds using open column chromatography from crude extract of S. trifasciata leaves that extracted by sonication method. There are six eluent used to isolate flavonoid which are methanol : ethyl acetate, chloroform : ethyl acetate, chloroform : ethyl acetate : methanol. Wilstater test is used to select the fraction that rich of flavonoid. The best result from isolation step that contains flavonoid is assessed the inhibitory activity of xanthine oxidase. It is analyzed qualitative using Liquid Chromatography Mass Spectrometry (LCMS). The inhibition percentage showed that fraction 3 was potential to inhibit XO by 85.48 %. LC-MS chromatogram can show that crude extract and positive fraction of isolation were containing falvonoid.This publication has 16 references indexed in Scilit:
- A new bioactive steroidal saponin from Sansevieria cylindricaPhytotherapy Research, 2003
- Pregnane glycosides from Sansevieria trifasciataPhytochemistry, 1997
- Steroidal saponins from Sansevieria trifasciataPhytochemistry, 1996
- Xanthine oxidase inhibitory activity of some Panamanian plants from Celastraceae and LamiaceaeJournal of Ethnopharmacology, 1995
- Xanthine Oxidase Inhibitors from the Leaves ofAlsophila Spinulosa(HOOK) TryonJournal of Enzyme Inhibition, 1994
- Xanthine oxidase inhibitory activity of Chilean MyrtaceaeJournal of Ethnopharmacology, 1991
- Phenolic constituents of licorice. II. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase.CHEMICAL & PHARMACEUTICAL BULLETIN, 1989
- Xanthine oxidase inhibitory activity of paraguayan myrtaceaeJournal of Ethnopharmacology, 1988
- Preliminary pharmacological studies on Eugenia uniflora leaves: Xanthine oxidase inhibitory activityJournal of Ethnopharmacology, 1987
- Flavonoids, a class of natural products of high pharmacological potencyBiochemical Pharmacology, 1983