Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision
- 1 June 2018
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 36 (6), 505-+
- https://doi.org/10.1038/nbt.4132
Abstract
We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.This publication has 15 references indexed in Scilit:
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