Ursolic acid enhances mouse liver regeneration after partial hepatectomy
- 2 December 2011
- journal article
- Published by Taylor & Francis Ltd in Pharmaceutical Biology
- Vol. 50 (4), 523-528
- https://doi.org/10.3109/13880209.2011.611143
Abstract
Context: Ursolic acid is a pentacyclic triterpenoid which has hepatoprotective and antihepatotoxic activities. Objective: This study investigated whether ursolic acid is able to stimulate liver regeneration in partially hepatectomized mice. Materials and methods: Ursolic acid or the vehicle solution was orally administered to the experimental, sham-operated and vehicle-treated group mice for 7 days, positive control animal (mice) was treated with recombinant human hepatocyte growth factor (rhHGF), and then the 70% liver partial hepatectomy was performed. The liver mass recovery rate was estimated by measuring the ratios of mice liver weight to body weight. The liver cells undergoing DNA synthesis were identified by immunohistochemistry analysis using monoclonal anti-BrdU antibodies. The expression levels of cyclin D1, cyclin E and C/EBP proteins (C/EBPα and C/EBPβ) were detected by the Western blotting technique. Results: Our results showed administration of ursolic acid significantly increased the ratio of the liver to body weight and BrdU labeling index at 36 and 48 h after partial hepatectomy, and the potency of UA is similar to rhHGF treated positive control mice. In addition, ursolic acid treatment significantly increased cyclin D1, cyclin E and C/EBPβ protein expression levels at 36 h after liver PHx compared with the vehicle-treated control mice. Discussion and conclusion: All these results suggest that ursolic acid stimulates liver proliferation after partial hepatectomy, and this effect may be associated with the stimulation of C/EBPβ expression.Keywords
This publication has 33 references indexed in Scilit:
- TEC protein tyrosine kinase is involved in the Erk signaling pathway induced by HGFBiochemical and Biophysical Research Communications, 2011
- The Age-Associated Decline of Glycogen Synthase Kinase 3β Plays a Critical Role in the Inhibition of Liver RegenerationMolecular and Cellular Biology, 2009
- Liver regenerationJournal of Cellular Physiology, 2007
- Antimutagenicity of ursolic acid and oleanolic acid against doxorubicin-induced clastogenesis in Balb/c miceLife Sciences, 2006
- Standardized assessment of cell proliferation: The approach of the RITA-CEPA working groupExperimental and Toxicologic Pathology, 2005
- Relationship between expression of cyclin D1 and impaired liver regeneration observed in fibrotic or cirrhotic ratsJournal of Gastroenterology and Hepatology, 2005
- c-Jun-N-terminal kinase drives cyclin D1 expression and proliferation during liver regenerationHepatology, 2003
- The mystery of liver regenerationBritish Journal of Surgery, 2002
- Lower Activity of CCAAT/Enhancer-Binding Protein and Expression of Cyclin E, but Not Cyclin D1, Activating Protein-1 and p21WAF1, after Partial Hepatectomy in Obstructive JaundiceBiochemical and Biophysical Research Communications, 2001
- CCAAT enhancer- binding protein beta is required for normal hepatocyte proliferation in mice after partial hepatectomy.JCI Insight, 1998