Methyl jasmonate increases silymarin production in Silybum marianum (L.) Gaernt cell cultures treated with β-cyclodextrins
- 25 September 2010
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 33 (1), 179-184
- https://doi.org/10.1007/s10529-010-0406-6
Abstract
Silymarin (Sm) from the fruit of Silybum marianum is an isomeric mixture of pharmacologically active flavonolignans which are formed by oxidative coupling of taxifolin (Tx) and coniferyl alcohol (CA). Suspension cultures of this plant constitutively secrete small amounts of Sm into the extracellular medium. Production can be increased by inclusion of cyclodextrins (CDs) in cultures. Both hydroxylated (RHCD) and dimethylated (RMCD) CDs strongly induced prompt accumulation of CA in the medium followed by a late production of flavonolignans. Simultaneous addition of methyl jasmonate (MJ) and RMCD to cells did not significantly modify CA release or flavonolignan accumulation. Delayed addition of MJ to cultures subcultivated in medium containing RMCD markedly influenced Sm production by promoting conversion of the previously formed CA precursor.Keywords
This publication has 18 references indexed in Scilit:
- Stimulation of menthol production in Mentha piperita cell cultureIn Vitro Cellular & Developmental Biology - Plant, 2008
- Synergistic effect of methyljasmonate and cyclodextrin on stilbene biosynthesis pathway gene expression and resveratrol production in Monastrell grapevine cell culturesBMC Research Notes, 2008
- Silybin and Silymarin - New and Emerging Applications in MedicineCurrent Medicinal Chemistry, 2007
- Modified Cyclodextrins Are Chemically Defined Glucan Inducers of Defense Responses in Grapevine Cell CulturesJournal of Agricultural and Food Chemistry, 2005
- Molecular Structure and Stereochemistry of Silybin A, Silybin B, Isosilybin A, and Isosilybin B, Isolated from Silybum marianum (Milk Thistle)Journal of Natural Products, 2003
- Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum)Electronic supplementary information (ESI) available: HPLC chromatograms of isolates and extracts. See http://www.rsc.org/suppdata/ob/b3/b300099k/Organic & Biomolecular Chemistry, 2003
- Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosumBiotechnology Letters, 2003
- Pharmacology of SilymarinClinical Drug Investigation, 2002
- Flavonolignan production from Silybum marianum transformed and untransformed root culturesFitoterapia, 2000
- Influence of medium composition on the accumulation of flavonolignans in cultured cells of Silybum marianum (L.) GaertnPlant Science, 1999