The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean
- 27 February 2008
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 146 (4), 1663-1672
- https://doi.org/10.1104/pp.108.115832
Abstract
The MADS-domain transcriptional regulator AGAMOUS-LIKE15 (AGL15) has been reported to enhance somatic embryo development when constitutively expressed. Here we report that loss-of-function mutants of AGL15, alone or when combined with a loss-of-function mutant of a closely related family member, AGL18, show decreased ability to produce somatic embryos. If constitutive expression of orthologs of AGL15 is able to enhance somatic embryo development in other species, thereby facilitating recovery of transgenic plants, then AGL15 may provide a valuable tool for crop improvement. To test this idea in soybean (Glycine max), a full-length cDNA encoding a putative ortholog of AGL15 was isolated from soybean somatic embryos. Subsequently, the corresponding genomic region of the gene was obtained. This gene, designated GmAGL15, encodes a protein with highest similarity to AGL15 from Arabidopsis (Arabidopsis thaliana) and Brassica napus that accumulates to its highest amount in embryos in these species. Like Arabidopsis and Brassica AGL15, GmAGL15 was preferentially expressed in developing embryos. When ectopically overexpressed the soybean protein was able to enhance somatic embryo development in soybean.Keywords
This publication has 32 references indexed in Scilit:
- TheArabidopsisSOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 Protein Complex Includes BRASSINOSTEROID-INSENSITIVE1Plant Cell, 2006
- Expression of MADS-box genes during the embryonic phase in ArabidopsisPlant Molecular Biology, 2005
- Control of expression and autoregulation of AGL15, a member of the MADS‐box familyThe Plant Journal, 2004
- AtGA3ox2, a Key Gene Responsible for Bioactive Gibberellin Biosynthesis, Is Regulated during Embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in ArabidopsisPlant Physiology, 2004
- The Transcription Factor FUSCA3 Controls Developmental Timing in Arabidopsis through the Hormones Gibberellin and Abscisic AcidDevelopmental Cell, 2004
- The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin MetabolismPlant Cell, 2004
- WUSCHEL induces shoot stem cell activity and developmental plasticity in the root meristemGenes & Development, 2004
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture.2001
- The MADS-Domain Protein AGAMOUS-Like 15 Accumulates in Embryonic Tissues with Diverse Origins1Plant Physiology, 1999