Oligothiophene-5-Labeled Deoxyuridines for the Detection of Single Nucleotide Polymorphisms
- 13 November 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 19 (1), 171-177
- https://doi.org/10.1021/bc7002555
Abstract
We report the synthesis of four oligothiophene-5-labeled deoxyuridines. These modified fluorescent nucleosides have been incorporated into oligodeoxynucleotides designed to be used as probes to discriminate, through changes in fluorescence emission, between hybridization with a perfectly complementary strand and that with a single nucleotide mismatch facing the modified uridines. Upon hybridization, remarkable differences (up to 47%) of the emitted light, depending on the uridine facing base, were observed.This publication has 23 references indexed in Scilit:
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