The immune response to herpes simplex virus: Comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes simplex virus type 1 infections
- 1 June 1985
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 16 (2), 147-162
- https://doi.org/10.1002/jmv.1890160207
Abstract
Employing an immunoblotting technique, the polypeptide specificity and relative titers of anti‐HSV IgG reactive with denaturation‐resistant epitopes on HSV proteins were determined in patients experiencing primary HSV‐1 infections at various anatomical sites. Early sera from previously seronegative patients with primary HSV‐1 infections were found to have comparatively low levels of antibody directed against the major viral glycoprotein antigens (gB, gC, and gD) relative to titers present in sera of individuals with long‐standing, latent orofacial HSV‐1 infections. Patients with primary infections did however have high titers of antibody directed against a series of low molecular weight HSV polypeptide antigens. These antigens were found to be antigenically related to a structural component of virion nucleocapsids. At later times postinfection, titers of antibodies directed against other viral polypeptides including the major glycoproteins increased to levels more closely approximating those observed in latently infected individuals. These results indicate that the anti‐HSV IgG detected by immunoblot analysis which appears earliest following primary infection is not directed against the known major infected cell or virion glycoprotein surface antigens but rather against an internal capsid protein of HSV.This publication has 59 references indexed in Scilit:
- Polypeptide Specificity of the Early Antibody Response Following Primary and Recurrent Genital Herpes Simplex Virus Type 2 InfectionsJournal of General Virology, 1984
- Effect of acyclovir treatment of primary genital herpes on the antibody response to herpes simplex virus.JCI Insight, 1984
- Relative Titers of Antibodies to Individual Polypeptide Antigens of Herpes Simplex Virus Type 1 in Human SeraThe Journal of Infectious Diseases, 1983
- Relationship between expression of herpes simplex virus glycoproteins and susceptibility of target cells to human natural killer activity.The Journal of Experimental Medicine, 1983
- Detection of IgE- and IgG-binding proteins after electrophoretic transfer from polyacrylamide gelsJournal of Immunological Methods, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Immunoprecipitation of herpes simplex virus type 1 antigens with different antisera and human cerebrospinal fluidsArchiv für die gesamte Virusforschung, 1977
- Detection of type-specific antibody to herpes simplex virus type 1 and 2 in human sera by complement-fixation testsArchiv für die gesamte Virusforschung, 1976
- Serologic Responses to Herpes Simplex Virus in Rabbits: Complement-requiring Neutralizing, Conventional Neutralizing, and Passive Hemagglutinating AntibodiesThe Journal of Infectious Diseases, 1974
- Type-specific protein in herpes simplex virus envelope reacts with neutralising antibodyNature, 1974