Embedding Media for 1–2 Micron Sectioning. 2. Hydroxyethyl Methacrylate Combined with 2-Butoxyethanol
- 1 January 1967
- journal article
- Published by Taylor & Francis Ltd in Stain Technology
- Vol. 42 (5), 253-255
- https://doi.org/10.3109/10520296709115020
Abstract
A hydroxyethyl methacrylate monomer medium incorporating 2-butoxyethanol requires 2 stock solutions for embedding. Solution A: 80 ml of hydroxyethyl methacrylate (Rohm and Haas Co., Philadelphia, Pa.) is mixed well with 16 ml of 2-butoxyethanol; 0.27 gm of benzoyl peroxide, the catalyst, is added and permitted to dissolve. Heating to 40–50 C may be used to accelerate its solution. Solution B: polyethylene glycol 200 or 400, 15 parts, and N,N-dimethylaniline, 1 part, are mixed thoroughly. Tissues are dehydrated in the customary manner to absolute ethanol or other comparable dehydrant, infiltrated completely with A, then cast in a mixture consisting of 42 parts of A well mixed with 1 part of B. Polymerizaion occurs in 4–7 hr. In a water bath at 20 C the time required was about 7 hr; at 28 C, 4 hr. This medium is based on the author's water-polyethylene glycol-hydroxyethyl methacrylate monomer medium (Stain Techn., 42: 119–23, 1967).Keywords
This publication has 2 references indexed in Scilit:
- Hydroxyethyl Methacrylate Combined with Polyethylene Glycol 400 and Water; an Embedding Medium for Routine 1–2 Micron SectioningStain Technology, 1967
- A METHOD FOR THE CYTOPHOTOMETRIC ESTIMATION OF RIBONUCLEIC ACIDJournal of Histochemistry & Cytochemistry, 1961