Differential Effects of Mild and Severe Cucumber mosaic virus Strains in the Perturbation of MicroRNA-Regulated Gene Expression in Tomato Map to the 3′ Sequence of RNA 2
Open Access
- 1 October 2009
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 22 (10), 1239-1249
- https://doi.org/10.1094/mpmi-22-10-1239
Abstract
Viral infections interfere with the microRNA (miRNA)-mediated regulation of gene expression, determining developmental defects. In tomato leaves, the accumulation levels of six miRNA species and their target transcripts corresponding to transcription factors with roles in plant development and leaf morphogenesis and two genes involved in the short RNA processing, DCL1 and AGO1, were significantly enhanced upon infection with the severe strain Cucumber mosaic virus (CMV)-Fny, while that of AGO4 was reduced. In plants harboring the infection of the mild strain CMV-LS, the effects on miRNA pathway were reduced, although AGO1, DCL1, and NAC1 also were shown to overaccumulate during infections exhibiting a mild phenotype. The use of the recombinant strain CMV-Fny(LS2b), in which the 3′-terminal region of CMV-Fny RNA 2, including the 2b coding sequence, was replaced with the corresponding region of CMV-LS RNA 2, provided evidence that the exchanged region was implicated in the perturbation of miRNA metabolism. In tomato plants infected with CMV-Fny supporting the ameliorative satellite (sat)RNA variant Tfn-satRNA, the symptomless phenotype correlated, with the exception of NAC1 upregulation, with the absence of effects on mitochondrial RNA and miRNA expression. Some of the aspects of miRNA pathway perturbation described were peculiar to CMV–tomato interactions and involved in the etiology of the disease phenotype elicited in this host.Keywords
This publication has 55 references indexed in Scilit:
- Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1Proceedings of the National Academy of Sciences of the United States of America, 2008
- Specific Enrichment of miRNAs in Arabidopsis thaliana Infected with Tobacco mosaic virusDNA Research, 2007
- The developmental selector AS1 is an evolutionarily conserved regulator of the plant immune responseProceedings of the National Academy of Sciences of the United States of America, 2007
- Specialization and evolution of endogenous small RNA pathwaysNature Reviews Genetics, 2007
- Conservation and evolution of miRNA regulatory programs in plant developmentCurrent Opinion in Plant Biology, 2007
- Infection and coaccumulation of tobacco mosaic virus proteins alter microRNA levels, correlating with symptom and plant developmentProceedings of the National Academy of Sciences of the United States of America, 2007
- Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomatoNature Genetics, 2007
- Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defenseGenes & Development, 2006
- Induction and suppression of RNA silencing: insights from viral infectionsNature Reviews Genetics, 2005
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001