Fluorescence-Based Nitric Oxide Detection by Ruthenium Porphyrin Fluorophore Complexes
- 8 September 2004
- journal article
- Published by American Chemical Society (ACS) in Inorganic Chemistry
- Vol. 43 (20), 6366-6370
- https://doi.org/10.1021/ic035418n
Abstract
The ruthenium(II) porphyrin fluorophore complexes [Ru(TPP)(CO)(Ds-R)] (TPP = tetraphenylporphinato dianion; Ds = dansyl; R = imidazole (im), 1, or thiomorpholine (tm), 2) were synthesized and investigated for their ability to detect nitric oxide (NO) based on fluorescence. The X-ray crystal structures of 1 and 2 were determined. The Ds-im or Ds-tm ligand coordinates to an axial site of the ruthenium(II) center through a nitrogen or sulfur atom, respectively. Both exhibit quenched fluorescence when excited at 368 or 345 nm. Displacement of the metal-coordinated fluorophore by NO restores fluorescence within minutes. These observations demonstrate fluorescence-based NO detection using ruthenium porphyrin fluorophore conjugates.This publication has 19 references indexed in Scilit:
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