Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique
Open Access
- 15 May 2013
- journal article
- video audio-media
- Published by MyJove Corporation in Journal of Visualized Experiments
- No. 75,p. e50172
- https://doi.org/10.3791/50172
Abstract
The forced oscillation technique (FOT) is a powerful, integrative and translational tool permitting the experimental assessment of lung function in mice in a comprehensive, detailed, precise and reproducible manner. It provides measurements of respiratory system mechanics through the analysis of pressure and volume signals acquired in reaction to predefined, small amplitude, oscillatory airflow waveforms, which are typically applied at the subject's airway opening. The present protocol details the steps required to adequately execute forced oscillation measurements in mice using a computer-controlled piston ventilator (flexiVent; SCIREQ Inc, Montreal, Qc, Canada). The description is divided into four parts: preparatory steps, mechanical ventilation, lung function measurements, and data analysis. It also includes details of how to assess airway responsiveness to inhaled methacholine in anesthetized mice, a common application of this technique which also extends to other outcomes and various lung pathologies. Measurements obtained in naïve mice as well as from an oxidative-stress driven model of airway damage are presented to illustrate how this tool can contribute to a better characterization and understanding of studied physiological changes or disease models as well as to applications in new research areas.Keywords
This publication has 27 references indexed in Scilit:
- Repeated invasive lung function measurements in intubated mice: an approach for longitudinal lung researchLaboratory Animals, 2011
- Effects of Central Airway Shunting on the Mechanical Impedance of the Mouse LungAnnals of Biomedical Engineering, 2010
- In vitro and in vivo assessment of pulmonary risk associated with exposure to combustion generated fine particlesEnvironmental Toxicology and Pharmacology, 2010
- Effect of severe calorie restriction on the lung in two strains of miceAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2008
- In Vivo-to-In Silico Iterations to Investigate Aeroallergen-Host InteractionsPLOS ONE, 2008
- Bronchodilatory effect of deep inspiration on the dynamics of bronchoconstriction in miceJournal of Applied Physiology, 2007
- Developmental changes in airway and tissue mechanics in miceJournal of Applied Physiology, 2005
- α1-Antitrypsin Determines the Pattern of Emphysema and Function in Tobacco Smoke–exposed MiceAmerican Journal of Respiratory and Critical Care Medicine, 2002
- Stability of methacholine chloride solutions under different storage conditions over a 9 month periodEuropean Respiratory Journal, 1998
- An analysis of pressure-volume characteristics of the lungsJournal of Applied Physiology, 1964