Neisseria meningitidis rifampicin resistant strains: analysis of protein differentially expressed
Open Access
- 24 September 2010
- journal article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 10 (1), 1-8
- https://doi.org/10.1186/1471-2180-10-246
Abstract
Background: Several mutations have been described as responsible for rifampicin resistance in Neisseria meningitidis. However, the intriguing question on why these strains are so rare remains open. The aim of this study was to investigate the protein content and to identify differential expression in specific proteins in two rifampicin resistant and one susceptible meningococci using two-dimensional electrophoresis (2-DE) combined with mass spectrometry. Results: In our experimental conditions, able to resolve soluble proteins with an isoelectric point between 4 and 7, twenty-three proteins have been found differentially expressed in the two resistant strains compared to the susceptible. Some of them, involved in the main metabolic pathways, showed an increased expression, mainly in the catabolism of pyruvate and in the tricarboxylic acid cycle. A decreased expression of proteins belonging to gene regulation and to those involved in the folding of polypeptides has also been observed. 2-DE analysis showed the presence of four proteins displaying a shift in their isoelectric point in both resistant strains, confirmed by the presence of amino acid changes in the sequence analysis, absent in the susceptible. Conclusions: The analysis of differentially expressed proteins suggests that an intricate series of events occurs in N. meningitidis rifampicin resistant strains and the results here reported may be considered a starting point in understanding their decreased invasion capacity. In fact, they support the hypothesis that the presence of more than one protein differentially expressed, having a role in the metabolism of the meningococcus, influences its ability to infect and to spread in the population. Different reports have described and discussed how a drug resistant pathogen shows a high biological cost for survival and that may also explain why, for some pathogens, the rate of resistant organisms is relatively low considering the widespread use of a particular drug. This seems the case of rifampicin resistant meningococci.Keywords
This publication has 24 references indexed in Scilit:
- Molecular characterization of resistance to rifampicin in clinical isolates of Neisseria meningitidisClinical Microbiology & Infection, 2009
- The cost of resistance to colistin in Acinetobacter baumannii: a proteomic perspectiveProteomics, 2009
- A Newly Identified 191A/C Mutation in the Rv2629 Gene that Was Significantly Associated with Rifampin Resistance in Mycobacterium tuberculosisJournal of Proteome Research, 2007
- The Role of Compensatory Mutations in the Emergence of Drug ResistancePLoS Computational Biology, 2006
- Rifampin-resistantNeisseria meningitidisEmerging Infectious Diseases, 2006
- The PEP–pyruvate–oxaloacetate node as the switch point for carbon flux distribution in bacteriaFEMS Microbiology Reviews, 2005
- Inventory of the Proteins inNeisseriameningitidisSerogroup B Strain MC58Journal of Proteome Research, 2005
- Rifampin-resistant Meningococcal DiseaseEmerging Infectious Diseases, 2005
- Description of new mutations in the rpoB gene in rifampicin-resistant Neisseria meningitidis selected in vitro in a stepwise mannerJournal of Medical Microbiology, 2003
- Probability-based protein identification by searching sequence databases using mass spectrometry dataElectrophoresis, 1999