Glycoconjugate and lipid components ofRhizobium"hedysari" IS123, a root-nodule symbiont of the stress-tolerant legumeHedysarum coronarium
- 1 April 1996
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 42 (4), 340-345
- https://doi.org/10.1139/m96-050
Abstract
We have examined the diversity of glycoconjugates and cellular lipids of Rhizobium "hedysari" IS123, a bacterial symbiont that specifically nodulates the drought-tolerant forage legume Hedysarum coronarium. IS123 develops a complete capsule consisting of a loose fibrillar network of ruthenium-red-staining acidic polymers and produces two different exopolysaccharides (EPS). EPS-A contains glucose, galactose, mannose, and a noncarbohydrate substitution tentatively identified as a lactyl ester. The composition of EPS-B, which includes glucose and galactose, as well as O-acetyl, pyruvyl, and succinyl substituents, is very similar to that of the EPS-II described in Rhizobium meliloti. IS123 also makes an O-acetylated heterooligosaccharide and unsubstituted β-1,2-glucans. The cellular fatty acid composition of IS123 is dominated by 18:1 and also includes 14:0, 16:0, 16:1, 3OH-16:0, 17:0Δ, 18:0, 3OH-18:0, and 19:0Δ. Phospholipids of IS123 include phosphatidylethanolamine, N-methyl phosphatidylethanolamine, N,N-dimethyl phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol.Key words: Rhizobium, capsule, exopolysaccharides, oligosaccharides, lipids.Keywords
This publication has 17 references indexed in Scilit:
- Characterization and symbiotic importance of acidic extracellular polysaccharides of Rhizobium sp. strain GRH2 isolated from acacia nodulesJournal of Bacteriology, 1993
- Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021Carbohydrate Research, 1990
- Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodulesJournal of Bacteriology, 1988
- Introduction ofRhizobium “hedysari”in alkaline clay‐loam soil by different inoculation techniquesArid Soil Research and Rehabilitation, 1988
- Transfer of the Rhizobium leguminosarum biovar trifolii symbiotic plasmid pRtr5a to a strain of Rhizobium sp. that nodulates on Hedysarum coronariumJournal of Applied Bacteriology, 1987
- Nitrogen fixation ability of exopolysaccharide synthesis mutants of Rhizobium sp. strain NGR234 and Rhizobium trifolii is restored by the addition of homologous exopolysaccharidesJournal of Bacteriology, 1987
- Symbiotic incompatibility between two forage species ofHedysarum, grown in Morocco, and their homologous rhizobiaFEMS Microbiology Letters, 1986
- Nodulation studies on legumes exotic to Australia:Hedysarum coronariumFEMS Microbiology Letters, 1984
- High‐Resolution 1H‐NMR Spectroscopy of Free and Glycosidically Linked O‐ Acetylated Sialic AcidsEuropean Journal of Biochemistry, 1982
- Composition of the Capsular and Extracellular Polysaccharides of Rhizobium japonicumPlant Physiology, 1980