Lost in Transgenesis
- 31 August 2012
- journal article
- review article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 111 (6), 761-777
- https://doi.org/10.1161/circresaha.111.262717
Abstract
The advent of modern mouse genetics has benefited many fields of diseased-based research over the past 20 years, none perhaps more profoundly than cardiac biology. Indeed, the heart is now arguably one of the easiest tissues to genetically manipulate, given the availability of an ever-growing tool chest of molecular reagents/promoters and “facilitator” mouse lines. It is now possible to modify the expression of essentially any gene or partial gene product in the mouse heart at any time, either gain or loss of function. This review is designed as a handbook for the nonmouse geneticist and/or junior investigator to permit the successful manipulation of any gene or RNA product in the heart, while avoiding artifacts. In the present review, guidelines, pitfalls, and limitations are presented so that rigorous and appropriate examination of cardiac genotype-phenotype relationships can be performed. This review uses examples from the field to illustrate the vast spectrum of experimental and design details that must be considered when using genetically modified mouse models to study cardiac biology.Keywords
This publication has 123 references indexed in Scilit:
- A Thrombospondin-Dependent Pathway for a Protective ER Stress ResponseCell, 2012
- Tbx20 regulation of cardiac cell proliferation and lineage specialization during embryonic and fetal development in vivoDevelopmental Biology, 2012
- A conditional knockout resource for the genome-wide study of mouse gene functionNature, 2011
- CIB1 is a regulator of pathological cardiac hypertrophyNature Medicine, 2010
- Heart-specific Deletion of CnB1 Reveals Multiple Mechanisms Whereby Calcineurin Regulates Cardiac Growth and FunctionOnline Journal of Public Health Informatics, 2010
- Cardiac-specific, inducible ClC-3 gene deletion eliminates native volume-sensitive chloride channels and produces myocardial hypertrophy in adult miceJournal of Molecular and Cellular Cardiology, 2010
- Isl1 is a direct transcriptional target of Forkhead transcription factors in second heart field-derived mesodermDevelopmental Biology, 2009
- Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injuryNature Medicine, 2007
- Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitorsProceedings of the National Academy of Sciences of the United States of America, 2007
- Islet 1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cellsDevelopmental Biology, 2007