Closing the lid on DNA end-to-end stacking interactions
- 2 June 2008
- journal article
- research article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 92 (22), 223901-2239013
- https://doi.org/10.1063/1.2937402
Abstract
Recent experiments suggest that short DNA strands associate by end-to-end stacking. Here, we report interactions between DNAs with modified ends. DNA duplexes, 20bp long, were capped with short T4 loops at 2, 1 or 0 ends, and were placed in solutions containing 20mM Mg2+. Association was observed only in constructs with one or more uncapped ends. DNA-DNA interactions were characterized by measuring variations in small angle x-ray scattering (SAXS) curves at the lowest scattering angles. Second virial coefficients were computed from the SAXS data. Our results confirm that end-to-end stacking plays an important role in short strand DNA-DNA interactions.Keywords
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