Multilaboratory Comparison of Growth Characteristics for Anaerobes, Using 5 Different Agar Media
Open Access
- 1 September 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Infectious Diseases
- Vol. 35 (s1), S36-S39
- https://doi.org/10.1086/341918
Abstract
A multilaboratory study compared the growth of 30 fastidious anaerobes, using 5 different agar media: Wilkins-Chalgren (WC), WC with either whole or laked sheep blood, and Brucella supplemented with vitamin K1 and hemin and either laked or whole sheep blood. The media were compared for quality and quantity of growth. Experiments were conducted either entirely in an anaerobic chamber or inoculated in ambient air with anaerobic incubation. The results showed that (1) any medium plus whole or laked blood was better than unsupplemented WC, (2) whole blood and laked blood additives gave similar results, (3) supplemented Brucella with whole or laked blood was superior to WC and WC with whole or laked blood, and (4) anaerobic and aerobic inoculation with anaerobic incubation gave similar results. Brucella agar supplemented with whole or laked blood supports the growth of fastidious anaerobic species better than the WC agars do.This publication has 3 references indexed in Scilit:
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- Collaborative Evaluation of a Proposed Reference Dilution Method of Susceptibility Testing of Anaerobic BacteriaAntimicrobial Agents and Chemotherapy, 1979
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