Monovalent fusion proteins of immunoglobulin E mimotopes are safe for therapy of type I allergy
- 17 September 2001
- journal article
- fj express-summary
- Published by Wiley in The FASEB Journal
- Vol. 15 (13), 2524-2526
- https://doi.org/10.1096/fj.00-0888fje
Abstract
By screening phage display random peptide libraries with purified immunoglobulin E (IgE) from birch pollen-allergic patients, we previously defined peptides mimicking natural IgE epitopes (mimotopes) of the major birch pollen allergen Bet v 1. The present study aimed to define a monovalent carrier for the IgE mimotopes to i nduce protective antibodies directed to the IgE epitopes, suitable for mimotope-specific therapy. We expressed the selected mimotopes as fusion proteins together with streptococcal albumin binding protein (ABP). The fusion proteins were recognized specifically by anti- Bet v 1 human IgE, which demonstrated that the mimotopes fused to ABP resemble the natural IgE epitope. Bet v 1-specific IgG was induced by immunization of BALB/c mice with fusion proteins. These IgG antibodies could inhibit IgE binding to Bet v 1. Skin testing of Bet v 1 allergic mice showed that the ABP mimotope constructs did not elicit type I skin reactions, although they possess IgE bindi ng structures. Our data suggest that IgE mimotopes are safe for epitope-specific immunotherapy of sensitized individuals, when presented in a monovalent form. Therefore, ABP-fused mimotopes are promising candidates for a new type of immunotherapy based on the precise induction of blocking antibodies.Keywords
This publication has 30 references indexed in Scilit:
- Nonallergenic Peptides from Surface-Exposed Areas or B-Cell Epitopes of Allergens for Specific ImmunotherapyInternational Archives of Allergy and Immunology, 2001
- Selection of an Immunogenic and Protective Epitope of the PsaA Protein ofStreptococcus pneumoniaeUsing a Phage Display LibraryHybridoma, 2000
- Immunologic characterization of monoclonal antibodies that modulate human IgE binding to the major birch pollen allergen Bet v 1Journal of Allergy and Clinical Immunology, 1997
- Modulation of the allergic immune response in BALB/c mice by subcutaneous injection of high doses of the dominant T cell epitope from the major birch pollen allergen Bet v 1Clinical and Experimental Immunology, 1997
- X-ray and NMR structure of Bet v 1, the origin of birch pollen allergyNature Structural & Molecular Biology, 1996
- IgE-producing hybridomas established after B-cell culture in the CD40 systemImmunology Letters, 1995
- Human anti‐IgE antibodies by repertoire cloningEuropean Journal of Immunology, 1994
- Mimicking of discontinuous epitopes by phage-displayed peptides, II. Selection of clones recognized by a protective monoclonal antibody against the Bordetella pertussis toxin from phage peptide librariesGene, 1993
- Selection of antibody ligands from a large library of oligopeptides expressed on a multivalent exposition vectorJournal of Molecular Biology, 1991
- Identification of Profilin as a Novel Pollen Allergen; IgE Autoreactivity in Sensitized IndividualsScience, 1991