DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum
Open Access
- 8 March 2006
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 100 (4), 728-738
- https://doi.org/10.1111/j.1365-2672.2006.02891.x
Abstract
Aims: The purpose of this study was to determine the global transcriptional response in a food-associated lactic acid bacterium during bile stress. Methods and Results: Clone-based DNA micro-arrays were employed to describe the global transcriptional response of Lactobacillus plantarum WCFS1 towards 0·1% porcine bile. Comparison of differential transcript profiles obtained during growth of Lact. plantarum on plates with and without bile revealed 28 and 62 putative genes, of which the expression was at least 2·5-fold up- or down-regulated by bile, respectively. Approximately, 50% of these genes appeared genetically linked, and 12 bile-responsive gene clusters were identified. Seven of the identified bile-responsive genes and gene clusters encode typical stress-related functions, including glutathione reductase and glutamate decarboxylase, involved in oxidative and acid stress, respectively. Moreover, 14 bile-responsive genes and gene clusters were identified that encode proteins that are located in the cell envelope, including the dlt operon and the F1F0 ATPase. Conclusions: The identification of a relatively high number of genes encoding cell envelope functions indicates a major impact of bile acids on the integrity and/or functionality of the cytoplasmic membrane and cell wall. Significance and Impact of the Study: The data presented here provide valuable clues towards the defence mechanisms that play a role during bile stress in Lact. plantarum.Keywords
This publication has 28 references indexed in Scilit:
- Quenching of microbial samples for increased reliability of microarray dataJournal of Microbiological Methods, 2006
- Glutathione Protects Lactococcus lactis against Oxidative StressApplied and Environmental Microbiology, 2003
- Bile salts and cholesterol induce changes in the lipid cell membrane of Lactobacillus reuteriJournal of Applied Microbiology, 2003
- Pharmacokinetics of Lactobacillus plantarum NCIMB 8826, Lactobacillus fermentum KLD, and Lactococcus lactis MG 1363 in the human gastrointestinal tractAlimentary Pharmacology & Therapeutics, 2000
- Establishment of conditions for green table olive fermentation at low temperatureInternational Journal of Food Microbiology, 1999
- The ftsH gene of Bacillus subtilis is involved in major cellular processes such as sporulation, stress adaptation and secretionMolecular Microbiology, 1997
- Plasmid profiles and curing of plasmids in Lactobacillus plantarum strains isolated from green olive fermentationsJournal of Applied Bacteriology, 1991
- The control and consequences of bacterial fermentation in the human colonJournal of Applied Bacteriology, 1991
- The Proton-Translocating ATPase Of Escherichia ColiAnnual Review of Biophysics and Biophysical Chemistry, 1990
- MICROBIAL ECOLOGY OF THE GASTROINTESTINAL TRACTAnnual Review of Microbiology, 1977