Proteomics and Its Role in Nutrition Research
Open Access
- 1 July 2006
- journal article
- research article
- Published by Elsevier BV in Journal of Nutrition
- Vol. 136 (7), 1759-1762
- https://doi.org/10.1093/jn/136.7.1759
Abstract
There are ∼100,000 proteins in humans with various physiological functions. The complement of proteins in the organism as well as their interactions is defined as the proteome. Its analysis (proteomics) by highly specific, sensitive, and accurate MS has been made possible with matrix-assisted laser desorption ionization or electrospray ionization of proteins and large peptides. Currently, the most commonly used proteomics technologies involve either specific digestion of proteins (the bottom-up approach using 2-dimensional polyacrylamide gel electrophoresis and multidimensional protein identification technology) or direct analysis of intact proteins after their chromatographic separation (the top-down approach and surface-enhanced laser desorption ionization). Proteomics holds great promise for discoveries in nutrition research, including profiles and characteristics of dietary and body proteins; digestion, absorption, and metabolism of nutrients; functions of nutrients and other dietary factors in growth, reproduction, and health; biomarkers of the nutritional status and disease; and individualized requirements of nutrients. The proteome analysis is expected to play an important role in solving major nutrition-associated problems in humans and animals, such as obesity, diabetes, cardiovascular disease, cancer, aging, and intrauterine fetal retardation.Keywords
This publication has 32 references indexed in Scilit:
- Nutrigenomics: The Impact of Biomics Technology on Nutrition ResearchAnnals of Nutrition and Metabolism, 2005
- Proteomics in nutrition research: principles, technologies and applicationsBritish Journal of Nutrition, 2005
- Bacterial response to eukaryotic cells: Analysis of differentially expressed proteins using nano liquid chromatography–electrospray ionization tandem mass spectrometryJournal of Chromatography A, 2005
- Dietary Effects of Copper and Iron Deficiency on Rat Intestine: A Differential Display Proteome AnalysisJournal of Proteome Research, 2005
- Proteomics of the rat gut: Analysis of the myenteric plexus‐longitudinal muscle preparationProteomics, 2005
- Gender-specific proteomic alterations in glycolytic and mitochondrial pathways in aging monkey heartsJournal of Molecular and Cellular Cardiology, 2004
- Role of Insulin Action and Cell Size on Protein Expression Patterns in AdipocytesPublished by Elsevier BV ,2004
- Hepatic protein expression of lean mice and obese diabetic mice treated with peroxisome proliferator‐activated receptor activatorsProteomics, 2003
- Characterization of transcription factors by mass spectrometry and the role of SELDI‐MSMass Spectrometry Reviews, 2002
- Large-scale analysis of the yeast proteome by multidimensional protein identification technologyNature Biotechnology, 2001