Differentiation of Mouse Erythroleukemia Cells Enhanced by Alternatively Spliced c- myb mRNA
- 14 September 1990
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 249 (4974), 1291-1293
- https://doi.org/10.1126/science.2205003
Abstract
C-myb, the normal cellular homolog of the retroviral transforming gene v-myb, encodes a nuclear, transcriptional regulatory protein (p75c-myb). C-myb is involved in regulating normal human hematopoiesis, and inhibits dimethyl sulfoxide-induced differentiation of Friend murine erythroleukemia (F-MEL) cells. An alternately spliced c-myb mRNA encodes a truncated version of p75c-myb (mbm2) that includes the DNA binding region and nuclear localization signal present in the c-myb protein, but does not contain the transcriptional regulatory regions. Constitutive expression of mbm2, in contrast to c-myb, here resulted in enhanced differentiation of F-MEL cells. These data suggest that the c-myb protooncogene encodes alternately spliced mRNA species with opposing effects on differentiation.Keywords
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