The mammary microenvironment alters the differentiation repertoire of neural stem cells
Open Access
- 30 September 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 105 (39), 14891-14896
- https://doi.org/10.1073/pnas.0803214105
Abstract
A fundamental issue in stem cell biology is whether adult somatic stem cells are capable of accessing alternate tissue sites and continue functioning as stem cells in the new microenvironment. To address this issue relative to neurogenic stem cells in the mouse mammary gland microenvironment, we mixed wild-type mammary epithelial cells (MECs) with bona fide neural stem cells (NSCs) isolated from WAP-Cre/Rosa26R mice and inoculated them into cleared fat pads of immunocompromised females. Hosts were bred 6–8 weeks later and examined postinvolution. This allowed for mammary tissue growth, transient activation of the WAP-Cre gene, recombination, and constitutive expression of LacZ. The NSCs and their progeny contributed to mammary epithelial growth during ductal morphogenesis, and the Rosa26-LacZ reporter gene was activated by WAP-Cre expression during pregnancy. Some NSC-derived LacZ+ cells expressed mammary-specific functions, including milk protein synthesis, whereas others adopted myoepithelial cell fates. Thus, NSCs and their progeny enter mammary epithelium–specific niches and adopt the function of similarly endowed mammary cells. This result supports the conclusion that tissue-specific signals emanating from the stroma and from the differentiated somatic cells of the mouse mammary gland can redirect the NSCs to produce cellular progeny committed to MEC fates.Keywords
This publication has 16 references indexed in Scilit:
- Alveolar progenitor cells develop in mouse mammary glands independent of pregnancy and lactationJournal of Cellular Physiology, 2007
- Interaction with the mammary microenvironment redirects spermatogenic cell fate in vivoProceedings of the National Academy of Sciences, 2007
- Parity-induced mammary epithelial cells are multipotent and express cell surface markers associated with stem cellsDevelopmental Biology, 2007
- Notch signalling regulates stem cell numbers in vitro and in vivoNature, 2006
- Paracrine signaling through the epithelial estrogen receptor α is required for proliferation and morphogenesis in the mammary glandProceedings of the National Academy of Sciences of the United States of America, 2006
- Parity-induced mouse mammary epithelial cells are pluripotent, self-renewing and sensitive to TGF-β1 expressionOncogene, 2004
- Single factors direct the differentiation of stem cells from the fetal and adult central nervous system.Genes & Development, 1996
- Experimental mammary epithelial morphogenesis in anin vivo model: Evidence for distinct cellular progenitors of the ductal and lobular phenotypeBreast Cancer Research and Treatment, 1996
- Mammary ductal elongation: Differentiation of myoepithelium and basal lamina during branching morphogenesisDevelopmental Biology, 1983
- Growth of Mouse Mammary Glands in vivo after Monolayer CultureScience, 1965