Evidence for Selection of 11 Amino Acid CDR3 Domains in VKIII-derived Immunoglobulin Light Chains in Kawasaki Disease
- 1 January 1997
- journal article
- research article
- Published by Informa UK Limited in Scandinavian Journal of Rheumatology
- Vol. 26 (5), 350-354
- https://doi.org/10.3109/03009749709065697
Abstract
Kawasaki disease (KD) is a rheumatic disease that occurs during childhood. Although T cells have been implicated as having an important role in the pathogenesis of KD, the role of B cells is unclear. To detect preferential expression of 11 amino acid complementarity determining region (CDR)3 domains, we used two-stage PCR (polymerase chain reaction) to analyze the CDR3 lengths of Vklll-derived immunoglobulin kappa light chains expressed in peripheral blood B cells during the acute, subacute, and convalescent phase of this disease. As controls, the peripheral blood B cells of age-matched normal and children with acute febrile diseases other than KD were tested. In 5 of 7 KD patients, expression of kappa light chains containing 11 amino acid codon CDR3 intervals was increased during the acute and subacute phase of KD but decreased during the convalescent phase. Two of the 7 KD patients showed the same pattern during the subacute and convalescent phase, but not during the acute phase. Two of the 5 patients with acute febrile diseases other than KD showed increased expression of K chains with 11 amino acid codon CDR3 intervals, but it was not a major fraction. Three of the 5 patients with acute febrile diseases other than KD and all normal control subjects showed only 9 and 10 amino acid CDR3 domains. These results strongly suggest that B cells expressing kappa light chains with the 11 amino acid CDR3 domains might be involved in the pathogenesis of KD.Keywords
This publication has 19 references indexed in Scilit:
- Evidence that the V kappa III gene usage is nonstochastic in both adult and newborn peripheral B cells and that peripheral CD5+ adult B cells are oligoclonal.JCI Insight, 1994
- Evidence of antigen receptor-influenced oligoclonal B lymphocyte expansion in the synovium of a patient with longstanding rheumatoid arthritis.JCI Insight, 1994
- Expressed human immunoglobulin ϰ genes and their hypermutationEuropean Journal of Immunology, 1993
- Human IgM+IgD+ B cells, the major B cell subset in the peripheral blood, express Vϰ genes with no or little somatic mutation throughout lifeEuropean Journal of Immunology, 1993
- The immunoglobulin kappa light chain repertoire expressed in the synovium of a patient with rheumatoid arthritisArthritis & Rheumatism, 1992
- Molecular analysis of the VxIII‐Jx junctional diversity of polyclonal rheumatoid factors during rheumatoid arthritis frequently reveals N additionEuropean Journal of Immunology, 1992
- Autoantibody-associated kappa light chain variable region gene expressed in chronic lymphocytic leukemia with little or no somatic mutation. Implications for etiology and immunotherapy.The Journal of Experimental Medicine, 1988
- Evidence for oligoclonal B cell expansion in the peripheral blood of patients with rheumatoid arthritis.Annals Of The Rheumatic Diseases, 1986
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Light chain heterogeneity of 19s and 7s anti‐γ‐globulins in rheumatoid arthritis and subacute bacterial endocarditisArthritis & Rheumatism, 1978