Repair of Traumatic Skeletal Muscle Injury with Bone-Marrow-Derived Mesenchymal Stem Cells Seeded on Extracellular Matrix
- 1 September 2010
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 16 (9), 2871-2881
- https://doi.org/10.1089/ten.tea.2009.0826
Abstract
Skeletal muscle injury resulting in tissue loss poses unique challenges for surgical repair. Despite the regenerative potential of skeletal muscle, if a significant amount of tissue is lost, skeletal myofibers will not grow to fill the injured area completely. Prior work in our lab has shown the potential to fill the void with an extracellular matrix (ECM) scaffold, resulting in restoration of morphology, but not functional recovery. To improve the functional outcome of the injured muscle, a muscle-derived ECM was implanted into a 1 × 1 cm2, full-thickness defect in the lateral gastrocnemius (LGAS) of Lewis rats. Seven days later, bone-marrow-derived mesenchymal stem cells (MSCs) were injected directly into the implanted ECM. Partial functional recovery occurred over the course of 42 days when the LGAS was repaired with an MSC-seeded ECM producing 85.4 ± 3.6% of the contralateral LGAS. This was significantly higher than earlier recovery time points (p < 0.05). The specific tension returned to 94 ± 9% of the contralateral limb. The implanted MSC-seeded ECM had more blood vessels and regenerating skeletal myofibers than the ECM without cells (p < 0.05). The data suggest that the repair of a skeletal muscle defect injury by the implantation of a muscle-derived ECM seeded with MSCs can improve functional recovery after 42 days.Keywords
This publication has 69 references indexed in Scilit:
- Vascular endothelial growth factor (VEGF) as a key therapeutic trophic factor in bone marrow mesenchymal stem cell-mediated cardiac repairBiochemical and Biophysical Research Communications, 2009
- Mesenchymal stem cells induce a weak immune response in the rat striatum after allo or xenotransplantationJournal of Cellular and Molecular Medicine, 2009
- Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a noninvasive therapeutic regimenAmerican Journal of Physiology-Heart and Circulatory Physiology, 2009
- Chemoattraction of Progenitor Cells by Remodeling Extracellular Matrix ScaffoldsTissue Engineering, Part A, 2009
- Dose–Response Relationship of Mesenchymal Stem Cell Transplantation and Functional Regeneration After Severe Skeletal Muscle Injury in RatsTissue Engineering, Part A, 2009
- Stem Cells for the Treatment of Skeletal Muscle InjuryClinics in Sports Medicine, 2009
- Optimizing Skeletal Muscle Reinnervation with Nerve TransferHand Clinics, 2008
- Autologous Bone Marrow–Derived Cultured Mesenchymal Stem Cells Delivered in a Fibrin Spray Accelerate Healing in Murine and Human Cutaneous WoundsTissue Engineering, 2007
- Bone marrow contribution to skeletal muscle: A physiological response to stressDevelopmental Biology, 2005
- Pluripotency of mesenchymal stem cells derived from adult marrowNature, 2002