Two new aflatoxin producing species, and an overview of Aspergillus section Flavi
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Open Access
- 1 June 2011
- journal article
- Published by Westerdijk Fungal Biodiversity Institute in Studies in Mycology
- Vol. 69 (1), 57-80
- https://doi.org/10.3114/sim.2011.69.05
Abstract
Aspergillus subgenus Circumdati section Flavi includes species with usually biseriate conidial heads, in shades of yellow-green to brown, and dark sclerotia. Several species assigned to this section are either important mycotoxin producers including aflatoxins, cyclopiazonic acid, ochratoxins and kojic acid, or are used in oriental food fermentation processes and as hosts for heterologous gene expression. A polyphasic approach was applied using morphological characters, extrolite data and partial calmodulin, β-tubulin and ITS sequences to examine the evolutionary relationships within this section. The data indicate that Aspergillus section Flavi involves 22 species, which can be grouped into seven clades. Two new species, A. pseudocaelatus sp. nov. and A. pseudonomius sp. nov. have been discovered, and can be distinguished from other species in this section based on sequence data and extrolite profiles. Aspergillus pseudocaelatus is represented by a single isolate collected from Arachis burkartii leaf in Argentina, is closely related to the non-aflatoxin producing A. caelatus, and produces aflatoxins B & G, cyclopiazonic acid and kojic acid, while A. pseudonomius was isolated from insects and soil in the USA. This species is related to A. nomius, and produces aflatoxin B1 (but not G-type aflatoxins), chrysogine and kojic acid. In order to prove the aflatoxin producing abilities of the isolates, phylogenetic analysis of three genes taking part in aflatoxin biosynthesis, including the transcriptional regulator aflR, norsolonic acid reductase and O-methyltransferase were also carried out. A detailed overview of the species accepted in Aspergillus section Flavi is presented.Keywords
This publication has 120 references indexed in Scilit:
- Comparison of Soil and Corn KernelAspergillus flavusPopulations: Evidence for Niche SpecializationPhytopathology®, 2011
- Cyclopiazonic Acid Biosynthesis of Aspergillus flavus and Aspergillus oryzaeToxins, 2009
- Mycotic Keratitis Due to Aspergillus nomiusJournal of Clinical Microbiology, 2009
- Genetic regulation of aflatoxin biosynthesis: From gene to genomeFungal Genetics and Biology, 2009
- Biological control of aflatoxin contamination of cropsJournal of Zhejiang University-SCIENCE B, 2008
- Case of Keratitis Caused by Aspergillus tamariiJournal of Clinical Microbiology, 2007
- Nonsporulating Clinical Isolate Identified as Petromyces alliaceus (Anamorph Aspergillus alliaceus ) by Morphological and Sequence-Based MethodsJournal of Clinical Microbiology, 2007
- Taxonomic revision of Aspergillus section Clavati based on molecular, morphological and physiological dataStudies in Mycology, 2007
- Polyphasic taxonomy of Aspergillus section Candidi based on molecular, morphological and physiological dataStudies in Mycology, 2007
- Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implicationsMycopathologia, 2006