Gene expression patterns of trembling aspen trees following long‐term exposure to interacting elevated CO2 and tropospheric O3
Open Access
- 8 April 2005
- journal article
- Published by Wiley in New Phytologist
- Vol. 167 (1), 129-142
- https://doi.org/10.1111/j.1469-8137.2005.01422.x
Abstract
• Expression of 4600 poplar expressed sequence tags (ESTs) was studied over the 2001–2002 growing seasons using trees of the moderately ozone (O3)‐tolerant trembling aspen (Populus tremuloides) clone 216 exposed to elevated CO2 and/or O3 for their entire 5‐yr life history. • Based on replication of the experiment in years 2001 and 2002, 238 genes showed qualitatively similar expression in at least one treatment and were retained for analysis. Of these 238 genes, 185 were significantly regulated (1.5‐fold) from one year to the other in at least one treatment studied. Less than 1% of the genes were regulated 2‐fold or more. • In the elevated CO2 treatment, relatively small numbers of genes were up‐regulated, whereas in the O3 treatment, higher expression of many signaling and defense‐related genes and lower expression of several photosynthesis and energy‐related genes were observed. Senescence‐associated genes (SAGs) and genes involved in the flavanoid pathway were also up‐regulated under O3, with or without CO2 treatment. Interestingly, the combined treatment of CO2 plus O3 resulted in the differential expression of genes that were not up‐regulated with individual gas treatments. • This study represents the first investigation into gene expression following long‐term exposure of trees to the interacting effects of elevated CO2 and O3 under field conditions. Patterns of gene‐specific regulation described in this study correlated with previously published physiological responses of aspen clone 216.Keywords
This publication has 82 references indexed in Scilit:
- Interacting elevated CO2 and tropospheric O3 predisposes aspen (Populus tremuloides Michx.) to infection by rust (Melampsora medusae f. sp. tremuloidae)Global Change Biology, 2002
- Heterologous Array Analysis in Pinaceae: Hybridization ofPinus taedacDNA Arrays with cDNA from Needles and Embryogenic Cultures ofP. taeda,P. sylvestrisorPicea abiesComparative and Functional Genomics, 2002
- Effects of elevated CO2 and O3 on aspen clones varying in O3 sensitivity: can CO2 ameliorate the harmful effects of O3?Environmental Pollution, 2001
- Fine-root biomass and fluxes of soil carbon in young stands of paper birch and trembling aspen as affected by elevated atmospheric CO2 and tropospheric O3Oecologia, 2001
- The effect of elevated carbon dioxide and ozone on leaf- and branch-level photosynthesis and potential plant-level carbon gain in aspenTrees, 2001
- Antioxidative systems, pigment and protein contents in leaves of adult mediterranean oak species (Quercus pubescens and Q. ilex) with lifetime exposure to elevated CO2New Phytologist, 1998
- Ozone and forest treesNew Phytologist, 1998
- MORE EFFICIENT PLANTS: A Consequence of Rising Atmospheric CO2?Annual Review of Plant Physiology and Plant Molecular Biology, 1997
- Interaction of elevated CO2 and O3 on growth, photosynthesis and respiration of three perennial species grown in low and high nitrogenPhysiologia Plantarum, 1996
- A simple and efficient method for isolating RNA from pine treesPlant Molecular Biology Reporter, 1993