Bleomycin resistance conferred by a drug‐binding protein
- 28 March 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 230 (1-2), 171-175
- https://doi.org/10.1016/0014-5793(88)80665-3
Abstract
The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E.coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein.Keywords
This publication has 17 references indexed in Scilit:
- Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1987
- LEU2 directed expression of ?-galactosidase activity and phleomycin resistance in Yarrowia lipolyticaCurrent Genetics, 1987
- Plasmid-determined bleomycin resistance in Staphylococcus aureusPlasmid, 1987
- Identification of the alkaline-labile product accompanying cytosine release during bleomycin-mediated degradation of d(CGCGCG)Journal of the American Chemical Society, 1986
- A new look at antibiotic resistanceFEMS Microbiology Letters, 1986
- Bleomycin resistance: a new dominant selectable marker for plant cell transformationPlant Molecular Biology, 1986
- Completion of the nucleotide sequence of the central region of Tn5confirms the presence of three resistance genesNucleic Acids Research, 1985
- The transposon Tn5 carries a bleomycin-resistance determinantGene, 1984
- Plasmid-Determined Resistance to Antimicrobial AgentsAnnual Review of Microbiology, 1978
- A new spectrophotometric assay for protein in cell extractsAnalytical Biochemistry, 1977