Lipid-bonded Conducting Polymer Layers for a Model Biomembrane: Application to Superoxide Biosensors

Abstract

Model biomembranes composed of poly-DATT/DGS/POPA and poly-DATT/DGS/CL were separately prepared on gold electrodes. A monolayer of 1,2-dioleoyl-sn-glycero-3-succinate (DGS) was covalently bonded onto electrochemically grown poly-(3,4-diamiono-2,2:5,2-terthiophene) (DATT) layers (thickness of ∼300 nm; particle size of ∼50 to 70 nm). The numbers of unit molecules of the poly-DATT layer and of the DGS immobilized onto the poly-DATT layers were 1.53 × 10^-7 and 1.56 × 10^-9 mol cm^-2, respectively, using a quartz crystal microbalance technique. The lipid bilayers of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate (POPA) and cardiolipin (CL) were formed onto the poly-DATT/DGS layer using the Langmuir−Blodgett technique. The surface characterizations of each step were investigated by SEM, AFM, and XPS analyses. Cytochrome c (cyt c) was immobilized onto these model biomembranes through the charge interaction between the positive charges of cyt c and the negative charges of phosphate groups in CL or POPA lipids. At the POPA- and CL-modified biomembranes, the formal potentials of the redox couple of the immobilized cyt c were 0.22 and 0.23 V (vs Ag/AgCl), respectively. The redox reaction of the immobilized cyt c at the POPA- and CL-modified biomembranes was quasireversible, and the electron-transfer rate constants were 0.121 s^-1 and 0.133 s^-1, respectively. The applicability of these cyt c immobilized bioimitation membranes as the biosensors was tested for the determination of superoxide.