Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus
- 2 September 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 101 (37), 13525-13530
- https://doi.org/10.1073/pnas.0405536101
Abstract
The general transcription factor TATA-binding protein (TBP) is a key initiation factor involved in transcription by all three eukaryotic RNA polymerases. In addition, the related metazoan-specific TBP-like factor (TLF/TRF2) is essential for transcription of a distinct subset of genes. Here we characterize the vertebrate-specific TBP-like factor TBP2, using in vitro assays, in vivo antisense knockdown, and mRNA rescue experiments, as well as chromatin immunoprecipitation. We show that TBP2 is recruited to promoters in Xenopus oocytes in the absence of detectable TBP recruitment. Furthermore, TBP2 is essential for gastrulation and for the transcription of a subset of genes during Xenopus embryogenesis. In embryos, TBP2 protein is much less abundant than TBP, and moderate overexpression of TBP2 partially rescues an antisense knockdown of TBP levels and restores transcription of many TBP-dependent genes. TBP2 may be a TBP replacement factor in oocytes, whereas in embryos both TBP and TBP2 are required even though they exhibit partial redundancy and gene selectivity.Keywords
This publication has 42 references indexed in Scilit:
- The genetics of TBP and TBP-related factorsTrends in Biochemical Sciences, 2003
- Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expressionGenes & Development, 2003
- Removing the Vertebrate-Specific TBP N Terminus Disrupts Placental β2m-Dependent Interactions with the Maternal Immune SystemCell, 2002
- IFN-Stimulated transcription through a TBP-free acetyltransferase complex escapes viral shutoffNature, 2002
- Spermiogenesis Deficiency in Mice Lacking the Trf2 GeneScience, 2001
- Distinct Roles for TBP and TBP-Like Factor in Early Embryonic Gene Transcription in XenopusScience, 2000
- Promoter-Selective Properties of the TBP-Related Factor TRF1Science, 2000
- DNA methylation directs a time-dependent repression of transcription initiationCurrent Biology, 1997
- Role for the Amino-Terminal Region of Human TBP in U6 snRNA TranscriptionScience, 1997
- Monoclonal Antibodies Directed against the Amino-Terminal Domain of Human TBP Cross-React with TBP from Other SpeciesHybridoma, 1996