Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH‐tolerant enzyme
Open Access
- 1 August 1993
- journal article
- Published by Wiley in JBIC Journal of Biological Inorganic Chemistry
- Vol. 216 (1), 155-160
- https://doi.org/10.1111/j.1432-1033.1993.tb18127.x
Abstract
The extracellular lipase of Bacillus subtilis 168 was purified from the growth medium of an overproducing strain by ammonium sulfate precipitation followed by phenyl-Sepharose and hy-droxyapatite column chromatography. The purified lipase had a strong tendency to aggregate. It exhibited a molecular mass of 19000 Da by SDS/PAGE and a pI of 9.9 by chromatofocusing. The enzyme showed maximum stability at pH 12 and maximum activity at pH 10. The lipase was active toward p-nitrophenyl esters and triacylglycerides with a marked preference for esters with C8 acyl groups. Using trioleyl glycerol as substrate, the enzyme preferantially cleaved the 1(3)-position ester bond. No interfacial activation effect was observed with triacetyl glycerol as substrate.Keywords
This publication has 19 references indexed in Scilit:
- Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solventNature, 1992
- Purification and properties of lipase from Penicillium simplicissimumBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Structure of human pancreatic lipaseNature, 1990
- A serine protease triad forms the catalytic centre of a triacylglycerol lipaseNature, 1990
- The molecular evolution of genes and proteins: a tale of two serinesNature, 1988
- Studies on pseudomonas fragi 22.39 b lipase. Part II Purification and some properties of lipase produced by Pseudomonas fragi 22.39B.Agricultural and Biological Chemistry, 1987
- Characterization of Signals Promoting Gene Expression on theStaphylococcus aureusPlasmid pUB110 and Development of a Gram-Positive Expression Vector SystemDNA, 1986
- Purification and General Properties of the Lipase of Thermophilic Fungus Humicola lanuginosa S-38Agricultural and Biological Chemistry, 1973
- Purification and Characterization of the Lipase of Pseudomonas fragiJournal of General Microbiology, 1967