Capacitive immunosensor for transferrin based on an o-aminobenzenthiol oligomer layer
- 6 May 2002
- journal article
- Published by Elsevier BV in Analytica Chimica Acta
- Vol. 458 (2), 297-304
- https://doi.org/10.1016/s0003-2670(02)00067-3
Abstract
A capacitance immunosensor based on an oligomer layer on gold was developed. o-Aminobenzenethiol (o-ABT) forms self-assembled layer (SAL) on polycrytalline gold surface. The SAL was polymerized electrochemically, then an oligomer of o-ABT was formed on the gold surface with some free-reacted ∼NH2 as functional groups to react with glutareldehyde (GA). The transferrin antiserum was covalently coupled on the gold surface by GA. The transferrin was determined with a detection limit of 0.12 ng/ml and a linear detection range of 1.25–80.0 ng/ml.Keywords
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