Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton
Open Access
- 12 April 2021
- journal article
- review article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
Lymphocytes rearrange their shape, membrane receptors and organelles during cognate contacts with antigen-presenting cells (APCs). Activation of T cells by APCs through pMHC-TCR/CD3 interaction (peptide-major histocompatibility complex-T cell receptor/CD3 complexes) involves different steps that lead to the reorganization of the cytoskeleton and organelles and, eventually, activation of nuclear factors allowing transcription and ultimately, replication and cell division. Both the positioning of the lymphocyte centrosome in close proximity to the APC and the nucleation of a dense microtubule network beneath the plasma membrane from the centrosome support the T cell’s intracellular polarity. Signaling from the TCR is facilitated by this traffic, which constitutes an important pathway for regulation of T cell activation. The coordinated enrichment upon T cell stimulation of the chaperonin CCT (chaperonin-containing tailless complex polypeptide 1; also termed TRiC) and tubulins at the centrosome area support polarized tubulin polymerization and T cell activation. The proteasome is also enriched in the centrosome of activated T cells, providing a mechanism to balance local protein synthesis and degradation. CCT assists the folding of proteins coming from de novo synthesis, therefore favoring mRNA translation. The functional role of this chaperonin in regulating cytoskeletal composition and dynamics at the immune synapse is discussed.Funding Information
- Ministerio de Ciencia e Innovación
This publication has 65 references indexed in Scilit:
- The Molecular Architecture of the Eukaryotic Chaperonin TRiC/CCTStructure, 2012
- A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cellsNature Immunology, 2011
- Asymmetric Proteasome Segregation as a Mechanism for Unequal Partitioning of the Transcription Factor T-bet during T Lymphocyte DivisionImmunity, 2011
- Motor-dependent microtubule disassembly driven by tubulin tyrosinationThe Journal of cell biology, 2009
- p90 Ribosomal S6 Kinase and p70 Ribosomal S6 Kinase Link Phosphorylation of the Eukaryotic Chaperonin Containing TCP-1 to Growth Factor, Insulin, and Nutrient SignalingOnline Journal of Public Health Informatics, 2009
- Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation.Cell Stress and Chaperones, 2009
- Defining the TRiC/CCT interactome links chaperonin function to stabilization of newly made proteins with complex topologiesNature Structural & Molecular Biology, 2008
- The interaction network of the chaperonin CCTThe EMBO Journal, 2008
- HEF1-Dependent Aurora A Activation Induces Disassembly of the Primary CiliumCell, 2007
- Centrosome biogenesis and function: centrosomics brings new understandingNature Reviews Molecular Cell Biology, 2007