Nocardioides marinus sp. nov.

Abstract

A Gram-positive, slightly halophilic, rod-shaped bacterium, designated CL-DD14^T, was isolated from seawater of the East Sea, Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CL-DD14^T belongs to the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between strain CL-DD14^T and the type strains of recognized species of the genus Nocardioides were low (94.1–97.1 %). Strain CL-DD14 ^T grew over the pH range 6–9 and temperature range 10–40 °C. The strain grew at NaCl concentrations of 0.5–8 % (w/v) with optimum growth at 1–3 % and no growth was observed after 3 weeks on nutrient agar without any salts. It contained ll-diaminopimelic acid as the diamino acid in the cell wall. The major isoprenoid quinone was MK-8(H₄) and the major cellular fatty acids were iso-C_16 : 0 and 10-methyl C_17 : 0. The DNA G+C content was 72.9 mol%. On the basis of phenotypic and phylogenetic data, strain CL-DD14^T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides marinus sp. nov. is proposed. The type strain is CL-DD14^T (=KCCM 42321^T=DSM 18248^T).