Expression of GPIV and Naka antigen on monocytes in Naka‐negative subjects whose platelets lack GPIV
- 1 July 1993
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 84 (3), 387-391
- https://doi.org/10.1111/j.1365-2141.1993.tb03091.x
Abstract
Summary. The platelet antigen Naka was once considered to be a platelet‐specific alloantigen and is carried on platelet membrane glycoprotein (GP) IV. Recent studies suggest that Naka‐negative subjects lack platelet GPIV. GPIV is an important adhesive receptor and expressed on the surface of monocytes as well as of platelets. In the present study, flow cytometry was used to detect GPIV and Naka antigen on the surface of monocytes. Naka antigen was expressed on monocytes as well as on platelets in Naka‐positive subjects (n= 6) (P‐GPIV‐positive subjects). To our surprise, monocytes of Naka‐negative subjects (n= 7) (P‐GPIV‐negative subjects) having no anti‐Naka antibody in their serum expressed GPIV and Naka antigen to almost the same degree as did the monocytes of P‐GPIV‐positive subjects. Competitive experiments using OKM5 (a monoclonal antibody against GPIV) and anti‐Naka antibody showed that the epitope of anti‐Naka antibody on monocytes was very close to that of OKM5. In two P‐GPIV‐negative subjects having anti‐Naka antibody in their serum, GPIV and Naka antigen were not expressed on the surface of either monocytes or platelets. These results indicate that the GPIV molecules and Naka antigen are expressed on the surface of monocytes in the majority of P‐GPIV‐negative subjects, but that in a very few P‐GPIV‐negative subjects neither GPIV nor Naka antigen is expressed on the surface of their monocytes. We hypothesize that P‐GPIV‐negative subjects who carry neither GPIV nor Naka antigen on their monocytes produce anti‐Naka antibody as a result of transfusion or pregnancy.Keywords
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