Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens
- 10 January 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Sensors
- Vol. 5 (1), 24-28
- https://doi.org/10.1021/acssensors.9b02137
Abstract
Due to its high carrier mobility, graphene is considered suitable material for use in field-effect transistors. However, its application to immunosensing of small molecules is still elusive. To investigate the potential of graphene field effect transistors (G-FET) as a sensor for small molecules with small or no charge, we applied the open-sandwich immunoassay (OS-IA), which detects low molecular weight antigens noncompetitively, to G-FET. Using an antibody variable fragment VL immobilized on graphene and a hyper acidic region of amyloid precursor protein fused to the other variable fragment VH, we successfully detected a small antigen peptide consisting of 7 amino acids (BGP-C7), with a more than 100-fold increase in sensitivity compared with that measured by enzyme-linked OS-IA. Furthermore, we succeeded in detecting BGP-C7 in the presence of human serum with similar sensitivity, suggesting its potential application in clinical diagnostics.Keywords
Funding Information
- Core Research for Evolutional Science and Technology (JPMJCR15F4)
- Japan Society for the Promotion of Science (JP17K06920, JP18H03851, JP19H02582)
- Japan Science and Technology Agency (JPMJMI18D9)
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