Enzyme, Protein, Carbohydrate, and Phenolic Contaminants in Commercial Tyrosinase Preparations: Potential Problems Affecting Tyrosinase Activity and Inhibition Studies
- 24 May 2008
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 56 (12), 4760-4768
- https://doi.org/10.1021/jf800109a
Abstract
Commercial mushroom tyrosinase contains other proteins, enzymes, carbohydrates, and phenolic material besides tyrosinase. Carbohydrate and phenolic material comprise a large percentage of the powder resuspensions derived from Agaricus bisporus. Enzyme assays identified the presence of tyrosinase, laccase, beta-glucosidase, beta-galactosidase, beta-xylosidase, cellulase, chitinase, xylanase, and mannanase in the commercial tyrosinase. Protein sequencing indicated the presence of tyrosinase, a lectin, and a putative mannanase as well as 10 unidentified protein/peptides in the commercial tyrosinase preparations. Characteristics of tyrosinase isoforms were similar in two different commercial tyrosinase sources. Inhibition studies indicated that I 50 values for some tyrosinase inhibitors were different when the crude powder was compared to a partially purified tyrosinase. The presence of these contaminants has the potential to affect studies using commercial tyrosinase.Keywords
This publication has 52 references indexed in Scilit:
- Hen egg white lysozyme as an inhibitor of mushroom tyrosinaseFEBS Letters, 2006
- A reliable reducing end assay for chito-oligosaccharidesCarbohydrate Polymers, 2004
- EFFECT OF SALICYLHYDROXAMIC ACID (SHAM) ON DL-DOPA OXIDATION BY MUSHROOM TYROSINASE AND BY NaIO4Journal of Food Biochemistry, 2000
- Characterisation of xylanases produced in liquid and compost cultures of the cultivated mushroom Agaricus bisporusMycological Research, 2000
- Tyrosinase activity and isoform composition in separate tissues during development of Agaricus bisporus fruit bodiesMycological Research, 1999
- Expression of CEL2 and CEL4, Two Proteins from Agaricus Bisporus with Similarity to Fungal Cellobiohydrolase I and -mannanase, Respectively, is Regulated by the Carbon SourceMicrobiology, 1997
- Tyrosinase and related proteins in mammalian pigmentationFEBS Letters, 1996
- Differentiation of Fungal Tyrosinases and Laccases Using Selective Inhibitors and SubstratesPublished by American Chemical Society (ACS) ,1995
- Tyrosinase, Laccase, and Peroxidase in Mushrooms (Agaricus, Crimini, Oyster, and Shiitake)Journal of Food Science, 1994
- Carbohydrate Metabolism in Agaricus bisporus (Lange) Sing.: Changes in Soluble Carbohydrates during Growth of Mycelium and SporophoreJournal of General Microbiology, 1976