Newcastle disease virus: Detection and characterization by PCR of recent German isolates differing in pathogenicity
Open Access
- 1 June 1998
- journal article
- research article
- Published by Taylor & Francis Ltd in Avian Pathology
- Vol. 27 (3), 237-243
- https://doi.org/10.1080/03079459808419330
Abstract
The fusion (F) protein plays an important role in determining the virulence of Newcastle disease virus (NDV) strains. A reverse transcriptase‐polymerase chain reaction (RT‐PCR) is described which amplifies a 362 bp fragment encompassing the region of the F protein most important for pathogenicity. A specific PCR product was obtained independent of strain, pathogenicity and host of origin. Sequencing of the region specifying the F protein cleavage site confirmed the correlation between deduced amino acid sequence and pathogenicity. Oligonucleotides corresponding to the sequence of the pathospecific region were designed for recent German NDV isolates and labelled with digoxigenin. Hybridization of PCR fragments of different isolates with pathotype‐specific oligonucleotides allowed an estimation of the pathogenicity of most isolates. Results were in good agreement with experimentally determined ICPI values.Keywords
This publication has 14 references indexed in Scilit:
- Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” based on the nucleotide sequence of the fusion protein geneArchiv für die gesamte Virusforschung, 1996
- Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F geneArchiv für die gesamte Virusforschung, 1996
- Evaluation of the molecular basis of pathogenicity of the variant Newcastle disease viruses termed ?pigeon PMV-1 viruses?Archiv für die gesamte Virusforschung, 1994
- Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicityArchiv für die gesamte Virusforschung, 1993
- Detection of Newcastle disease virus RNA in infected allantoic fluids by in vitro enzymatic amplification (PCR)Archiv für die gesamte Virusforschung, 1991
- Structure, Function, and Intracellular Processing of the Glycoproteins of ParamyxoviridaePublished by Springer Science and Business Media LLC ,1991
- Nucleotide Sequence of the Fusion and Haemagglutinin-Neuraminidase Glycoprotein Genes of Newcastle Disease Virus, Strain Ulster: Molecular Basis for Variations in Pathogenicity between StrainsJournal of General Virology, 1988
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- The Spread of a Pathogenic and an Apathogenic Strain of Newcastle Disease Virus in the Chick Embryo as Depending on the Protease Sensitivity of the Virus GlycoproteinsJournal of General Virology, 1979
- Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virusVirology, 1976