Activation of tyrosine kinase of EGFR induces Gβγ‐dependent GRK–EGFR complex formation
- 30 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (1), 122-126
- https://doi.org/10.1016/j.febslet.2004.11.057
Abstract
This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. The EGF-induced GRK2-EGFR complex formation was greatly reduced by perturbation of EGFR and Src tyrosine kinase activity. Furthermore, studies with GRK2 mutants showed that neither catalytic activity nor the N-terminal domain of GRK2 was required for EGF-induced GRK2-EGFR complex formation. However, overexpression of Gbetagamma scavengers blocked EGF-induced formation of GRK2-EGFR complex.Keywords
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