Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds
- 24 February 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 561 (1-3), 127-131
- https://doi.org/10.1016/s0014-5793(04)00148-6
Abstract
The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16-ABI5 triggered growth retardation and ABA-independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signalingKeywords
This publication has 20 references indexed in Scilit:
- Regulation of storage protein gene expression inArabidopsisDevelopment, 2003
- ABI5 acts downstream of ABI3 to execute an ABA‐dependent growth arrest during germinationThe Plant Journal, 2002
- Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress ResponsePlant Physiology, 2002
- The Homologous ABI5 and EEL Transcription Factors Function Antagonistically to Fine-Tune Gene Expression during Late EmbryogenesisPlant Cell, 2002
- Changes in gene expression in the leafy cotyledon1 (lec1) and fusca3 (fus3) mutants of Arabidopsis thaliana L.Journal of Experimental Botany, 2000
- The Arabidopsis Abscisic Acid Response Gene ABI5 Encodes a Basic Leucine Zipper Transcription FactorPlant Cell, 2000
- Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid.Plant Cell, 1994
- The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformationPlant Molecular Biology, 1994
- Mutations at two new Arabidopsis ABA response loci are similar to the abi3 mutationsThe Plant Journal, 1994
- Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.Genes & Development, 1988