Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin
- 1 January 1985
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 313 (5998), 144-147
- https://doi.org/10.1038/313144a0
Abstract
Epidermal growth factor (EGF), through interaction with specific cell surface receptors, generates a pleiotropic response that, by a poorly defined mechanism, can induce proliferation of target cells. Subversion of the EGF mitogenic signal through expression of a truncated receptor may be involved in transformation by the avian erythroblastosis virus (AEV) oncogene v-erb-B, suggesting that similar EGF receptor defects may be found in human neoplasias. Overexpression of EGF receptors has been reported on the epidermoid carcinoma cell line A431, in various primary brain tumours and in squamous carcinomas. In A431 cells the receptor gene is amplified. Here we show that 4 of 10 primary brain tumours of glial origin which express levels of EGF receptors that are higher than normal also have amplified EGF receptor genes. Amplified receptor genes were not detected in the other brain tumours examined. Further analysis of EGF receptor defects may show that such altered expression and amplification is a particular feature of certain human tumours.Keywords
This publication has 25 references indexed in Scilit:
- Gene amplification in cultured animal cellsCell, 1984
- Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cellsNature, 1984
- Expression Cloning of Human EGF Receptor Complementary DNA: Gene Amplification and Three Related Messenger RNA Products in A431 CellsScience, 1984
- Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cellsNature, 1984
- Amplification and Enhanced Expression of the Epidermal Growth Factor Receptor Gene in A431 Human Carcinoma CellsScience, 1984
- Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequencesNature, 1984
- The erbB gene of avian erythroblastosis virus is a member of the src gene familyCell, 1983
- Regulation of Cell Proliferation by Epidermal Growth FactorCritical Reviews in Biochemistry, 1983
- onc gene amplification in promyelocytic leukaemia cell line HL-60 and primary leukaemic cells of the same patientNature, 1982
- Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell lineNature, 1982