The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion
Open Access
- 14 October 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), 2723
- https://doi.org/10.1038/srep02723
Abstract
Plant roots consist of a meristematic zone of mitotic cells and an elongation zone of rapidly expanding cells, in which DNA replication often occurs without cell division, a process known as endoreduplication. The duration of the cell cycle and DNA replication, as measured by 5-ethynyl-2′-deoxy-uridine (EdU) incorporation, differed between the two regions (17 h in the meristematic zone, 30 h in the elongation zone). Two distinct subnuclear patterns of EdU signals, whole and speckled, marked nuclei undergoing DNA replication at early and late S phase, respectively. The boundary region between the meristematic and elongation zones was analysed by a combination of DNA replication imaging and optical estimation of the amount of DNA in each nucleus (C-value). We found a boundary cell with 4C nuclei exhibiting the whole pattern of EdU signals. Analyses of cells in the boundary region revealed that endoreduplication precedes rapid cell elongation in roots.This publication has 45 references indexed in Scilit:
- JAGGED Controls Growth Anisotropy and Coordination between Cell Size and Cell Cycle during Plant OrganogenesisCurrent Biology, 2012
- Control of Drosophila endocycles by E2F and CRL4CDT2Nature, 2011
- Light-Dependent Regulation ofDEL1Is Determined by the Antagonistic Action of E2Fb and E2FcPlant Physiology, 2011
- Model-Based Analysis of Arabidopsis Leaf Epidermal Cells Reveals Distinct Division and Expansion Patterns for Pavement and Guard CellsPlant Physiology, 2011
- Programmed induction of endoreduplication by DNA double-strand breaks in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2011
- Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marksNature Structural & Molecular Biology, 2011
- CULLIN 4-RING FINGER-LIGASE plays a key role in the control of endoreplication cycles in Arabidopsis trichomesProceedings of the National Academy of Sciences of the United States of America, 2010
- Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulatorsProceedings of the National Academy of Sciences of the United States of America, 2009
- Extreme polyploidy in a large bacteriumProceedings of the National Academy of Sciences of the United States of America, 2008
- A chemical method for fast and sensitive detection of DNA synthesis in vivoProceedings of the National Academy of Sciences of the United States of America, 2008