Altered estrogen receptor expression in skeletal muscle and adipose tissue of female rats fed a high-fat diet
- 1 April 2011
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 110 (4), 1046-1053
- https://doi.org/10.1152/japplphysiol.00541.2010
Abstract
Estrogen receptors (ERs) are expressed in adipose tissue and skeletal muscle, with potential implications for glucose metabolism and insulin signaling. Previous studies examining the role of ERs in glucose metabolism have primarily used knockout mouse models of ERα and ERβ, and it is unknown whether ER expression is altered in response to an obesity-inducing high-fat diet (HFD). The purpose of the current study was to determine whether modulation of glucose metabolism in response to a HFD in intact and ovariectomized (OVX) female rats is associated with alterations in ER expression. Our results demonstrate that a 6-wk HFD (60% calories from fat) in female rats induces whole body glucose intolerance with tissue-specific effects isolated to the adipose tissue, and no observed differences in insulin-stimulated glucose uptake, GLUT4, or ERα protein expression levels in skeletal muscle. In chow-fed rats, OVX resulted in decreased ERα with a trend toward decreased GLUT4 expression in adipose tissue. Sham-treated and OVX rats fed a HFD demonstrated a decrease in ERα and GLUT4 in adipose tissue. The HFD also increased activation of stress kinases (c-jun NH2-terminal kinase and inhibitor of κB kinase β) in the sham-treated rats and decreased expression of the protective heat shock protein 72 (HSP72) in both sham-treated and OVX rats. Our findings suggest that decreased glucose metabolism and increased inflammation in adipose tissue with a HFD in female rats could stem from a significant decrease in ERα expression.Keywords
This publication has 47 references indexed in Scilit:
- Obesity induced by high-fat diet promotes insulin resistance in the ovaryJournal of Endocrinology, 2010
- Impaired oxidative metabolism and inflammation are associated with insulin resistance in ERα-deficient miceAmerican Journal of Physiology-Endocrinology and Metabolism, 2010
- Leptin Contributes to the Adaptive Responses of Mice to High-Fat Diet Intake through Suppressing the Lipogenic PathwayPLOS ONE, 2009
- Heat Treatment Improves Glucose Tolerance and Prevents Skeletal Muscle Insulin Resistance in Rats Fed a High-Fat DietDiabetes, 2009
- Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed miceAmerican Journal of Physiology-Endocrinology and Metabolism, 2008
- HSP72 protects against obesity-induced insulin resistanceProceedings of the National Academy of Sciences of the United States of America, 2008
- Reversal of diet‐induced insulin resistance with a single bout of exercise in the rat: the role of PTP1B and IRS‐1 serine phosphorylationJournal Of Physiology-London, 2006
- A central role for JNK in obesity and insulin resistanceNature, 2002
- Insulin signalling and the regulation of glucose and lipid metabolismNature, 2001
- Chronic High-Fat Feeding and Middle-Aging Reduce in an Additive Fashion Glut4 Expression in Skeletal Muscle and Adipose TissueBiochemical and Biophysical Research Communications, 1997