Changes in the Enzymes for Fatty Acid Synthesis and Desaturation during Acclimation of Developing Soybean Seeds to Altered Growth Temperature
Open Access
- 1 June 1989
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 90 (2), 760-764
- https://doi.org/10.1104/pp.90.2.760
Abstract
Temperature-induced changes in the enzymes for fatty acid synthesis and desaturation were studied in developing soybean seeds (Glycine max L. var Williams 82). Changes were induced by culture of the seed pods for 20 hours in liquid media at 20, 25, or 35°C. Linoleoyl and oleoyl desaturases were 94 and 10 times as active, respectively, in seeds cultured at 20°C as those cultured at 25°C. Both desaturases had negligible activity in seeds cultured at 35°C compared to seeds cultured at 20°C. Though less dramatic, other enzymes also showed differences in activity after 20 hours in culture at 20, 25, or 35°C. Stearoyl-acyl carrier protein (ACP) desaturase and CDP-choline:diacylglycerol phosphorylcholine transferase were most active in preparations from 20°C cultures. Activities were twofold lower at 25°C and a further threefold lower in 35°C cultures. Cultures from 25 and 35°C had 60 and 40%, respectively, of the phosphorylcholine:CTP cytidylyl transferase activity present in cultures grown at 20°C. Fatty acid synthetase, malonyl-coenzyme A:ACP transacylase, palmitoyl-ACP elongation, and choline kinase were not significantly altered by culture temperature. These data suggest that the enzymes for fatty acid desaturation and phosphatidylcholine synthesis can be rapidly modulated in response to altered growth temperatures, while the enzymes for fatty acid synthesis and elongation are not.Keywords
This publication has 16 references indexed in Scilit:
- Phosphatidylcholine synthesis in castor bean endosperm: Characteristics and reversibility of the choline kinase reactionArchives of Biochemistry and Biophysics, 1988
- Phosphatidylcholine synthesis in castor bean endosperm: The localization and control of CTP:Choline-phosphate cytidylyltransferase activityArchives of Biochemistry and Biophysics, 1987
- High-performance liquid chromatographic analysis of serum long-chain fatty acids by direct derivatization methodJournal of Chromatography B: Biomedical Sciences and Applications, 1987
- A Comparison of Oleic Acid Metabolism in the Soybean (Glycine max [L.] Merr.) Genotypes Williams and A5, a Mutant with Decreased Linoleic Acid in the SeedPlant Physiology, 1986
- Partial purification and characterization of two forms of malonyl-coenzyme A:Acyl carrier protein transacylase from soybean leaf tissueArchives of Biochemistry and Biophysics, 1986
- Metabolism and control of lipid structure modificationBiochemistry and Cell Biology, 1986
- Low-temperature-induced changes in intracellular fatty acid fluxes in Dunaliella salinaArchives of Biochemistry and Biophysics, 1985
- [41] Acyl carrier protein from Escherichia coliMethods in enzymology, 1981
- Phosphatidylcholine Synthesis in Castor Bean EndospermPlant Physiology, 1976
- Fat metabolism in higher plantsArchives of Biochemistry and Biophysics, 1974