Human intestinal tissue tropism in Escherichia coli O157 : H7 – initial colonization of terminal ileum and Peyer's patches and minimal colonic adhesion ex vivo
- 1 March 2007
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 153 (3), 794-802
- https://doi.org/10.1099/mic.0.2006/003178-0
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) are an important cause of diarrhoeal and renal disease in man. Studies of a single prototypic O157 : H7 strain have shown tropism for follicle-associated epithelium (FAE) of distal ileal Peyer's patches without colonization of either small or large intestine. This study determined tropism in a range of Shiga toxin (Stx)-negative EHEC strains and looked for factors that might induce colonic colonization using human in vitro intestinal organ culture (IVOC). An FAE-restricted colonization was confirmed in two strains; four strains additionally colonized ileal villous surfaces, and adhesion to proximal small intestinal FAE was observed. All strains showed minimal adhesion to non-FAE regions of proximal small intestinal and to the transverse colon. Extensive large-bowel IVOC studies using three O157 : H7 strains, an O26 : H11 and an O103 : H2 strain, and tissue from caecum to rectum found colonization and attaching/effacing lesion formation in only 4 of 113 (3.5 %) IVOCs. Colonic adhesion was not enhanced by altering the IVOC technique or environment. Co-incubation of O157 : H7-infected ileal FAE with colonic samples enhanced colonic colonization, producing a novel, non-intimate adhesive phenotype. Thus, in the initial stages of colonization Stx-negative EHEC preferentially infect FAE and villi of the terminal ileal region ex vivo; colonic colonization is infrequently observed as an initial event but may represent a subsequent stage of infection.Keywords
This publication has 40 references indexed in Scilit:
- Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFP), EspA filaments and intiminMicrobiology, 2004
- Human intestinal tissue tropism of intimin epsilon O103Escherichia coliFEMS Microbiology Letters, 2003
- Tissue Tropism of Enteropathogenic Escherichia coli Strains Belonging to the O55 SerogroupInfection and Immunity, 2002
- Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7Gut, 2002
- Detection of Intimins α, β, γ, and δ, Four Intimin Derivatives Expressed by Attaching and Effacing Microbial PathogensJournal of Clinical Microbiology, 1998
- Pathogenesis of O157:H7 Escherichia Coli Infection in Neonatal CalvesAdvances in experimental medicine and biology, 1997
- The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches.Proceedings of the National Academy of Sciences of the United States of America, 1996
- Inoculum Size in Shigellosis and Implications for Expected Mode of TransmissionThe Journal of Infectious Diseases, 1989
- Diarrhea Due to Escherichia coli Strain RDEC-1 in the Rabbit: The Peyer's Patch as the Initial Site of Attachment and ColonizationThe Journal of Infectious Diseases, 1981
- Number, size, and distribution of Peyer's patches in the human small intestine: Part I The development of Peyer's patchesGut, 1965