A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human δ and β globin genes
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (22), 5931-5948
- https://doi.org/10.1093/nar/9.22.5931
Abstract
A new class of human interspersed repeated sequences distinct from the AluI family was found by screening a human gene library with a mouse ribosomal gene non-transcribed spacer probe (rDNA NTS). A member of this sequence family was localized to a 251 bp segment between the human delta and beta globin genes: a region previously judged to be devoid of repeated DNA. The complete nucleotide sequence of this segment revealed a tandem block of 17 TG dinucleotides, a feature hypothesized by others to be a recombination hot spot responsible for gene conversion in the gamma globin locus region. When the genomes of Xenopus, pigeon, slime mold and yeast were examined, reiterated sequences homologous to both the mouse rDNA NTS and human globin repeat were found in every case. The discovery of this extraordinarily conserved repeated sequence family appears to have depended upon not using salmon sperm DNA during hybridization. The use of eucaryotic carrier DNA may bias the search for repeated sequences against any which may be highly conserved during eucaryotic evolution.Keywords
This publication has 38 references indexed in Scilit:
- Repeated Genes in EukaryotesAnnual Review of Biochemistry, 1980
- Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequencesNature, 1980
- The genetic behaviour of a cloned mouse ribosomal DNA segment mimics mouse ribosomal gene evolutionJournal of Molecular Biology, 1979
- A ubiquitous family of repeated DNA sequences in the human genomeJournal of Molecular Biology, 1979
- Unique arrangement of coding sequences for 5 S, 5.8 S, 18 S and 25 S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysisJournal of Molecular Biology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Homology relationship between the messenger RNA and heterogenous nuclear RNA of mouse L cells. A DNA excess hybridization studyJournal of Molecular Biology, 1977
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966
- Specific duplex formation in vitro of mammalian DNAJournal of Molecular Biology, 1965