Derivatization-GAS Chromatographic Determination of Perindopril

Abstract

A capillary gas chromatographic method is described for the determination of perindopril, an anti-hypertensive agent. The method is based on the derivatization of perindopril with pentafluorobenzyl bromide in acetone, using potassium carbonate as a base catalyst. Under a mild condition, the highly polar and nonvolatile perindopril was derivatized into a derivative with suitable chromatographic properties. The resulting derivative was separated on an SE-54 capillary column and detected by a flame-ionization detector. Several parameters affecting the derivatization of perindopril were investigated. The linear range of the method for the determination of perindoplil was over 20–300 nmol. The detection limit (signal to noise ratio of 5) of perindopril in 0.2 mL acetone solution was about 3 nmol. Application of the method to the analysis of perindopril in tablets proved to be feasible.