Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults
Open Access
- 1 February 2007
- journal article
- research article
- Published by Elsevier BV in The American Journal of Clinical Nutrition
- Vol. 85 (2), 504-510
- https://doi.org/10.1093/ajcn/85.2.504
Abstract
Background: Cruciferous vegetable (CV) consumption is associated with a reduced risk of several cancers in epidemiologic studies. Objective: The aim of this study was to determine the effects of watercress (a CV) supplementation on biomarkers related to cancer risk in healthy adults. Design: A single-blind, randomized, crossover study was conducted in 30 men and 30 women (30 smokers and 30 nonsmokers) with a mean age of 33 y (range: 19–55 y). The subjects were fed 85 g raw watercress daily for 8 wk in addition to their habitual diet. The effect of supplementation was measured on a range of endpoints, including DNA damage in lymphocytes (with the comet assay), activity of detoxifying enzymes (glutathione peroxidase and superoxide dismutase) in erythrocytes, plasma antioxidants (retinol, ascorbic acid, α-tocopherol, lutein, and β-carotene), plasma total antioxidant status with the use of the ferric reducing ability of plasma assay, and plasma lipid profile. Results: Watercress supplementation (active compared with control phase) was associated with reductions in basal DNA damage (by 17%; P = 0.03), in basal plus oxidative purine DNA damage (by 23.9%; P = 0.002), and in basal DNA damage in response to ex vivo hydrogen peroxide challenge (by 9.4%; P = 0.07). Beneficial changes seen after watercress intervention were greater and more significant in smokers than in nonsmokers. Plasma lutein and β-carotene increased significantly by 100% and 33% (P < 0.001), respectively, after watercress supplementation. Conclusion: The results support the theory that consumption of watercress can be linked to a reduced risk of cancer via decreased damage to DNA and possible modulation of antioxidant status by increasing carotenoid concentrations.Keywords
This publication has 40 references indexed in Scilit:
- Dietary Lutein and Zeaxanthin: Possible Effects on Visual FunctionPublished by Oxford University Press (OUP) ,2005
- Chemoprevention by isothiocyanates and their underlying molecular signaling mechanismsMutation Research, 2004
- Plasma levels of six carotenoids in nine European countries: report from the European Prospective Investigation into Cancer and Nutrition (EPIC)Public Health Nutrition, 2004
- Cruciferous vegetable consumption alters the metabolism of the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in humansCarcinogenesis: Integrative Cancer Research, 2004
- Green vegetable drink consumption protects peripheral lymphocytes DNA damage in Korean smokersBioFactors, 2004
- Relation of Vegetable, Fruit, and Grain Consumption to Colorectal Adenomatous PolypsAmerican Journal of Epidemiology, 1996
- Effects of consumption of Brussels sprouts on intestinal and lymphocytic glutathione S-transferases in humansCarcinogenesis: Integrative Cancer Research, 1995
- Reduction of oxidative DNA-damage in humans by Brussels sproutsCarcinogenesis: Integrative Cancer Research, 1995
- A simple technique for quantitation of low levels of DNA damage in individual cellsExperimental Cell Research, 1988
- Glucosinolates and their breakdown products in food and food plantsC R C Critical Reviews in Food Science and Nutrition, 1983