Hepatitis C virus productive infection in mononuclear cells from patients with cryoglobulinaemia
Open Access
- 20 December 2006
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 147 (2), 241-248
- https://doi.org/10.1111/j.1365-2249.2006.03272.x
Abstract
The relationship between the occurrence of cryoglobulins and hepatitis C virus (HCV) productive infection in peripheral blood and bone marrow-derived lymphocytes was explored. HCV minus strand RNA, the viral replicative intermediate, was searched for by a polyA+ tract strand-specific Tth-based reverse transcriptase–polymerase chain reaction (RT–PCR) in lymphoid cells of 46 patients with acute and chronic infection. The HCV minus strand was demonstrated in RNA extracted from six (13%) and five (11%) peripheral blood and bone marrow-derived lymphocytes, respectively. The HCV replicating form in lymphoid cells was associated strictly with mixed cryoglobulinaemia (MCG), in that it was found in six of 13 (46%) MCG patients, including two with B cell non-Hodgkin's lymphoma (NHL). No traces of HCV-negative strand RNA were found in four patients with acute hepatitis C, in 15 with chronic active hepatitis without extrahepatic disorders, in seven with monoclonal gammopathy of undetermined significance, and in seven with B-NHL without MCG. These results emphasize the direct role of the virus in the pathogenesis of MCG and support the contention that HCV is not specifically lymphotropic, its entry and replication in lymphoid cells being determined largely by selective interactions.Keywords
This publication has 35 references indexed in Scilit:
- Productive Replication of Hepatitis C Virus in Perihepatic Lymph Nodes In Vivo: Implications of HCV LymphotropismGastroenterology, 2006
- Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered EntryJournal of Virology, 2006
- HCV-associated B cell clonalities in the liver do not carry the t(14;18) chromosomal translocationHepatology, 2005
- Second hepatitis C replication compartment indicated by viral dynamics during liver transplantationJournal of Hepatology, 2005
- Mutations in the putative HCV‐E2 CD81 binding regions and correlation with cell surface CD81 expressionJournal of Viral Hepatitis, 2004
- Intrahepatic B cell clonal expansions and extrahepatic manifestations of chronic HCV infectionEuropean Journal of Immunology, 2003
- Antiviral Treatment Down-Regulates Peripheral B-Cell CD81 Expression and CD5 Expansion in Chronic Hepatitis C Virus InfectionJournal of Virology, 2003
- Specific detection of minus-strand hepatitis C virus RNA by reverse-transcription polymerase chain reaction on polyA+-purified RNAHepatology, 2000
- Histological grading and staging of chronic hepatitisJournal of Hepatology, 1995
- Specific detection of positive and negative stranded hepatitis C viral RNA using chemical RNA modificationArchiv für die gesamte Virusforschung, 1994